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ROTI®Pol TaqS Mix (2x), 2 ml, 2 x 1 ml

2x conc., ready-to-use
Icon_Eisstern All products identified as being particularly temperaturesensitive are shipped in special ice boxes with freezer packs or in dry ice.
Master mix Taq DNA polymerase, recombinant, PCR master mix
Density (D) 1 g/cm³
Storage temp. -20 °C
Transport temp. cooled

Taq master mix for all standard PCR amplifications.
PCR premix w/o primers and template, optimized for reactions of 25 µl volume.

For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.

49,35 €/VE 

Excl. btw | 2 ml Per VE

Bestelnr. 9239.1

Op voorraad
Vrij van verzendingskosten vanaf 250 €
Snelle en betrouwbare levering!
Vanaf 6 VE 46,88 €/VE
Vanaf 24 VE 44,42 €/VE
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For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.

ROTI®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.

ROTI®Pol TaqS Mix (2x) 2x conc., ready-to-use

Optimized pre-mixed 2x PCR solution containing the recombinant TaqS DNA polymerase from the thermophilic bacterium Thermus aquaticus, dNTPs, MgCl2, and all other components required for PCR except primers and template DNA. ROTI®Pol TaqS Mix (2x) is recommended for use in all standard PCR applications.

PCR assaying with ROTI®Pol TaqS Mix (2x) master mix not only reduces contamination risks, but is also time-saving, highly reproducible and very easy to prepare. ROTI®Pol TaqS Mix (2x) can be applied to all standard Taq-based cycling protocols and is, therefore, the optimal choice for high throughput PCR as being performed in, for instance, analysis of cloning efficiency or colony screening, for routine screening processes, and for student's courses.

Due to the optimized composition of the master mix, the TaqS polymerase delivers specific PCR amplification of good yield with a wide range of PCR templates. ROTI®Pol TaqS Mix (2x) is able to amplify PCR products up to 3 kb with genomic DNA, and is appropriate for use with pure DNA solutions, cDNA, and bacterial colonies as templates. The use of the colourless PCR Master mix is particularly recommended when direct fluorescence or absorbance readings are required. The TaqS polymerase included in the master mix possesses a 5’ → 3’ polymerase- as well as a 5’-flap endonuclease activity, and generates a 3’dA (adenine)-overhang which may well be used for TA-cloning purposes.

Figure: Sensitivity assay using ROTI®Pol TaqS, TaqHY, Hot-TaqS and Hot-TaqHY, 1 U/reaction each (20 μl). 300 bp β-Actin fragment, 40 cycles. Template: 10 to 2000 pg human gDNA. Gel loading 10 μl each.
M: 100 bp-DNA Ladder extended. NTC: no template control.

De set bevat

2 or 10 vials, respectively, with 1,25 ml each of ROTI®Pol TaqS Mix (2x) containing TaqS polymerase, 0,4 mM each dNTP, and 4 mM MgCl2 in 2x reaction buffer

Geen medisch hulpmiddel / Geen ivD-product
Technische informatie
Toepassing Standard PCR 
Amplicon-einden 3'dA 
Master-mix colourless 
Polymerase Standard Taq polymerase 
Toepassing Standard PCR 
ROTI®Pol TaqS Mix (2x)
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Verpakking Prijs Hoeveelheid
9239.1 2 ml plastic 2 x 1 ml

49,35 €

9239.2 10 ml plastic 10 x 1 ml

198,90 €

Op voorraad
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS


Hier kunt u uw analysecertificaat voor het geselecteerde product zoeken en downloaden. Geef een chargenummer op.
De volgende analysecertificaten werden gevonden:

Type analysis

Endonuclease activitynone detected
Exonuclease activitynone detected
Suitability for PCR (400 bp)complies
Suitability for PCR (3 kb)complies
Selective specificity in PCRcomplies