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Proteinase K, 500 mg, 1 x 500 mg

≥30 mAnson U/mg, BioAnalysis Grade, lyophilized
Icon_DNAse-free
Icon_RNase-free
Mogelijke gevolgen: leiden tot schade aan de gezondheid, irriteren organen, huid of luchtwegen. Leiden in grotere hoeveelheden tot de dood. Veiligheid: zoals hierboven; bij huidirritaties of oogcontact met water of geschikt middel spoelen.
Mogelijke gevolgen: veroorzaken allergieën, kankerverwekkend (carcinogeen), verandering erfelijk materiaal (mutageen), brengt de voortplanting in gevaar en vruchtbeschadigend (reprotoxisch) of orgaanbeschadigend Veiligheid: voor het werk met dergelijke stoffen moet men zich goed informeren; veiligheidskleding en handschoenen, oog- en mondbescherming of adembescherming dragen.
Danger
H315-H317-H319-H334-H335
i causes skin irritation, may cause an allergic skin reaction, causes serious eye irritation, may cause allergy or asthma symptoms or breathing difficulties if inhaled, may cause respiratory irritation
P260 P280 P342+P311
i do not breathe dust, wear protective gloves/eye protection, if experiencing respiratory symptoms: Call a POISON CENTER/doctor
Verwante producten
VE
Verp.
Verpakking
Endopeptidase
Storage temp. +4 °C
WGK 1
CAS No. 39450-01-6
EG-Nr. 254-457-8

Highly pure serine protease for degrading proteins in biological samples. Isolated from fungi (Tritirachium album).
Stock solution: 20 mg/ml in water. Storage temperature (stock solution): -20 °C.
Art. No. 7528.3: A The stock solution (20 mg/ml) can be prepared directly in the bottle.

Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.
Productdetails
407,45 € /VE

325,90 €/VE  Campaign price!

Excl. btw | 500 mg Per VE

Bestelnr. 7528.2

Op voorraad
Vrij van verzendingskosten vanaf 250 €
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Productdetails


Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.


Gebruiksinstructies

Foreign activity: RNAse and DNAse not detectable
Optimum temperature: +65 °C.
Activity at +65 °C is ca. 12 x higher than at +25 °C. Over +65 °C, inactivation due to denaturation.
Activators: Denaturating agents like SDS (0,5-1 %), urea.
Inhibitors: Inhibition with Hg2+-ions, DFP, PMSF and phenol. Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotryps ininhibitors.
Stability: pH 4.0-12.5. pH optimum: 8,0.
Also stable even when denaturing agents, e.g. SDS and urea are present.
Stabilisers: Ca2+-ions (1-5 mM) prevent autolysis.



Proteinase K ≥30 mAnson U/mg, BioAnalysis Grade, lyophilized

A non-specific endopeptidase with strong proteolytic activity for degrading proteins in biological samples. A quality product for molecular biology with a broad scope of application.


Gebruiksinstructies

Working solution: 50 µg/ml
Reaction buffer: 50 mM Tris-HCl; pH 7.5; 5 mM CaCl2; 0.5 % SDS



Proteinase K
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Verpakking Prijs Hoeveelheid
7528.1 100 mg glass 1 x 100 mg
120,30 €

96,20 €

7528.2 500 mg glass 1 x 500 mg
407,45 €

325,90 €

7528.3 500 mg glass in 30 ml glass vial
446,15 €

356,90 €

7528.4 1 g glass 1 x 1 g
671,90 €

537,50 €

7528.5 250 mg glass 1 x 250 mg
224,70 €

179,70 €

7528.6 5 g glass 1 x 5 g
2.682,15 €

2.145,70 €

Op voorraad
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In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS


Algemene informatie

Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)

There are six classes in which all enzymes are classified according to the particular reaction they catalyse:

Oxidoreductases (catalyse redox reactions)

Transferases (transfer functional groups among substrates)

Hydrolases (cleave bonds via addition of water)

Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)

Isomerases (transform chemical isomers)

Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)


Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)

There are six classes in which all enzymes are classified according to the particular reaction they catalyse:

Oxidoreductases (catalyse redox reactions)

Transferases (transfer functional groups among substrates)

Hydrolases (cleave bonds via addition of water)

Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)

Isomerases (transform chemical isomers)

Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)


Analysecertificaten

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De volgende analysecertificaten werden gevonden:

Guarantee analysis

Activity (Hemoglobin, 37 °C)≥30 U/mg
DNases, RNasesnot detected
Endonucleasesnot detected
Assay DNA≤10 pg/mg
Solubility (c=20 mg/ml in H2O)clear, colorless