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ROTIPHORESE®5x TBE Buffer, 1 l, glass
Boiling point (bp) 100 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
WGK 1
TBE-buffer for preparing sequencing gels and as running buffer in electrophoresis.
30,65 €/VE
Excl. btw | 1 l Per VE
Bestelnr. 1L9K.1
Geschikt voor / Toebehoren
Productdetails
The TBE buffer is optimally suited as a gel and running buffer system.
TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels.
ROTIPHORESE®TBE buffer has a high buffer capacity and is therefore ideally suited as a gel and running buffer system for sequencing gels.
ROTIPHORESE®5x TBE Buffer ROTIPHORESE® 5x conc.
ROTIPHORESE®5x TBE buffer contains 0,5 M Tris-Borate pH 8,3 and 10 mM EDTA in distilled, deionised water; pH 8,3.
Suitable for manufacturing sequencing gels with ROTIPHORESE®Gel 40 and as a running buffer.
ROTIPHORESE®TBE buffer is usually used as 1x or 0.5-fold concentrated solution. TBE buffer is particularly suitable for the separation of small DNA fragments in the gel (up to 3 kb). TBE buffer is not suitable for use of gels for gel extraction of DNA.
TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels.
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Bestelnr. | VE | Verp. | Prijs | Hoeveelheid | |
---|---|---|---|---|---|
1L9K.1 | 1 l | glass |
30,65 € |
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1L9K.2 | 2,5 l | glass |
66,15 € |
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1L9K.3 | 5 l | plastic |
107,00 € |
|
|
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- Tussentotaal: 0.00
Downloads / MSDS
Analysecertificaten
Composition
Appearance | clear, colorless solution |
0.5 M TRIS borate (pH 8.3), 10 mM EDTA., distilled, deionized water., Remark: The product may crystallise. Can be redissolved by heating (max. 40 °C). |