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HEPES, 1 kg
Molar mass (M) 238,31 g/mol
Flash point (flp) 124 °C
Melting point (mp) 212,6 °C
WGK 1
CAS No. 7365-45-9
EG-Nr. 230-907-9
321,45 €/VE
Excl. btw | 1 kg Per VE
Bestelnr. 6763.3
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Productdetails
Free acid
HEPES ≥99,5 %, BioScience Grade
HEPES is a versatile buffer, which is zwitterionic over a broad pH-range, interesting for biological assays, and, therefore, doesn’t pass through biological membranes. In contrast to NaHCO3 buffers, it also doesn’t release any CO2 which affects the surroundings and has to be compensated. Hence, HEPES is particularly adapted for cell- and tissue cultures.
HEPES reacts with DEPC. It's recommended to use DNase/RNase/Protease free HEPES BioScience-Grade as soon as molecular biological applications like PCR, extraction, isolation, hybridization etc. are to be performed.
Buffer reagent according to Good. High buffer capacity at physiological pH-values.
pH-bereik | 6.8 tot 8.2 |
Bufferreagens | N-2-Hydroxyethyl piperazine-N'-2-ethane sulphonic acid, Good's buffer |
- Tussentotaal: 0.00
Bestelnr. | VE | Verp. | Prijs | Hoeveelheid | |
---|---|---|---|---|---|
6763.1 | 100 g | plastic |
47,85 € |
|
|
6763.2 | 500 g | plastic |
188,15 € |
|
|
6763.3 | 1 kg | plastic |
321,45 € |
|
|
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- Tussentotaal: 0.00
Downloads / MSDS
Analysecertificaten
Guarantee analysis
Assay | ≥99,5 % |
Solubility (0,1 M in H2O) | clear, complete |
pKa (at 25 °C) | 7,45-7,65 |
Absorbance | |
260 nm | ≤0,02 |
280 nm | ≤0,02 |
405 nm | ≤0,01 |
Chloride (Cl) | ≤0,005 % |
Sulphate (SO4) | ≤0,01 % |
Phosphate (PO4) | ≤0,001 % |
Calcium (Ca) | ≤0,003 % |
Sodium (Na) | ≤0,005 % |
Heavy metals (as Pb) | ≤0,0005 % |
DNases, RNases, Protease | none detected |