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Phenylmethyl sulphonyl fluoride (PMSF), 5 g, plastic

≥99 %, for biochemistry
Icon_EVE-EUD
Mogelijke gevolgen: vernietigen metalen en zijn bijtend voor lichaamsweefsel; ernstig oogletsel is mogelijk. Veiligheid: contact vermijden; veiligheidsbril en handschoenen dragen. Bij contact ogen en huid met water spoelen.
Mogelijke gevolgen: leiden in kleinere hoeveelheden onmiddellijk tot ernstige schade aan de gezondheid of tot de dood. Veiligheid: niet inademen, aanraken, inslikken. Beschermingsmiddelen dragen. Onmiddellijk antigifcentrum of arts op de hoogte brengen. Stabiele zijpositie.
danger
H301-H314
i toxic if swallowed, causes severe skin burns and eye damage
P280 P301+P330+P331 P303+P361+P353 P305+P351+P338 P310.0
i wear protective gloves/protective clothing/eye protection/face protection, IF SWALLOWED: rinse mouth. Do NOT induce vomiting, IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water [or shower], IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, immediately call a POISON CENTER/doctor
Verp.
Pack.
α-Toluene sulphonyl fluoride, PMSF
Empirical formula C7H7FO2S
Molar mass (M) 174,2 g/mol
Density (D) 0,79 g/cm³
Melting point (mp) 94 °C
ADR 8 II
WGK 1
CAS No. [329-98-6]
EG-Nr. 206-350-2
UN-Nr. 2923

Irreversible inhibitor of serine- and cysteine proteases.
Stock solution: 17.4 mg/ml (100 mM) in isopropanol, ethanol or methanol.
Storage of stock solution protected from light at –20 °C.

For your convenience, Art. No. 6367.4 comes packed in a 30 ml brown glass bottle. Addition of 20 ml 2-propanol results in a 100 mM stock solution.

General, irreversible inhibitor of serin proteases (chymotrypsin, trypsin and thrombin) and for the  ...
Productdetails
Type analysis

31,10 €/VE 

Excl. btw | 5 g Per VE

Bestelnr. 6367.1

In stock
Vrij van verzendingskosten vanaf 125 €
24-Uur verzending
Vanaf 6 VE 29,54 €/VE
Vanaf 24 VE 27,99 €/VE
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Productdetails



Phenylmethyl sulphonyl fluoride (PMSF) ≥99 %, for biochemistry

General, irreversible inhibitor of serin proteases (chymotrypsin, trypsin and thrombin) and for the dystin-protease papain. Inhibiton by sulphonation of the serine localised in the enzymatic active site.

Reversible inhibitor of cysteine-proteases. Ki = 0.91 ±0.01 mM


Working concentration: 17–174 µg/ml (0,1–1 mM).
Dissolves easily in isopropanol, ethanol and methanol. Not stable in aqueous solutions (half-life value of 1 h at pH 7.5/RT).
For stock solutions of 200-250 mM heat shortly (approx. 30 mins) to 30 °C.
Stock solutions should be stored at -20 °C. Short term storage of few weeks may be done at +4 °C in the dark.
PMSF reacts with thiols. DTT and β-mercaptoethanol reduce the inhibitory effect.
At every stage of isolating or purifying the proteins, PMSF should be added fresh from the stock solution. PMSF is often not sufficient as the only protease inhibitor in the preparation to protect against decomposition processes, as for example high salt concentration (3.0 M) impairs the inhibitory effect of PMSF.



Phenylmethyl sulphonyl fluoride (PMSF)
Geselecteerde hoeveelheid:   0
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Bestelnr. Verp. Pack. Prijs Hoeveelheid
6367.1 5 g plastic 31,10 €
6367.2 25 g plastic 103,10 €
6367.3 100 g plastic 289,20 €
6367.4 348 mg glass 14,55 €
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Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

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Analysecertificaten

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De volgende analysecertificaten werden gevonden:

Type analysis

Appearancecolourless crystals
Assay (GC)≥99 %
Identity (Raman)complies
Melting point91-95 °C
Solubility in ethanolcomplies
Solubility in methanolcomplies

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