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Transfer membrane ROTI®NC 0.45, 400 × 30 cm

Van  Carl ROTH
Materiaal: nitrocellulose (100 %).
Verwante producten
Afmeting


Transfer membrane for protein analyses, Southern- and Northern-Blots.

High-quality transfer membrane for all detection systems - extremely suitable for all protein transfer and detection methods. The membrane stands out for its particularly high mechanical stability. The pure 100 % nitrocellulose quality membrane is easy to block and allows for background-free blots with perfect bands.
Productdetails

492,35 €/VE 

Excl. btw | 1 rol(len) Per VE

Bestelnr. 9201.1

Niet meer verkrijgbaar
Vrij van verzendingskosten vanaf 250 €
Vanaf 6 VE 467,73 €/VE
Vanaf 24 VE 443,12 €/VE

Productdetails

Materiaal: nitrocellulose (100 %).

Transfer membrane for protein analyses, Southern- and Northern-Blots.

High-quality transfer membrane for all detection systems - extremely suitable for all protein transfer and detection methods. The membrane stands out for its particularly high mechanical stability. The pure 100 % nitrocellulose quality membrane is easy to block and allows for background-free blots with perfect bands.



Transfer membrane ROTI®NC 0.45 

Technische informatie
Afmeting 400 × 30 cm 
Uitvoering roll 
Binding hydrophobic 
Bindingscapaciteit 100 µg/cm²
Immobilisering UV, baking (max. 80 °C) 
Transfer membrane ROTI®NC 0.45
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. Afmeting VE Prijs Hoeveelheid
9201.1 400 × 30 cm 1 rol(len)

492,35 €

9200.1 300 × 30 cm 1 rol(len)

385,95 €

Op voorraad
Beschikbaar
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS

Geen documenten beschikbaar

Algemene informatie

Technical Parameters of ROTH-Transfer Membranes

Membrane Material Characteristics Thickness (μm) Binding capacity (μg/cm2) Pore size (μm) Immobilisation
Roti® Fluoro PVDF Polyvinyliden fluoride hydrophobic 125 ±25 300 0,2 not necessary
Transfer membrane ROTI®PVDF 0.2 Polyvinyliden fluoride hydrophobic 190 ±50 ≥125 0,2 not necessary
Transfer membrane ROTI®PVDF 0.45 Polyvinyliden fluoride hydrophobic 190 ±50 ≥125 0,45 not necessary
Immobilon P Polyvinyliden fluoride hydrophobic 120 ±25 150-300 0,45 not necessary
Roti® Nylon 0.2 Nylon 6,6 neutral/amphoteric 150 ±20 500 0,2/0,45 UV, baking
Roti® Nylon plus Nylon 6,6 hydrophilic, positively charged 160 ±20 500 0,45 not necessary, UV and baking possible
Transfer membrane ROTI®NC 0.2 Nitrocellulose hydrophobic 150 ±50 100 0,2 UV, baking (max. 80 °C)
Transfer membrane ROTI®NC 0.45 Nitrocellulose hydrophobic 150 ±50 100 0,45 UV, baking (max. 80 °C)
Amersham Protran® Nitrocellulose electrostatic, hydrophobic unknown 11-72 0,2/0,45 UV, baking (max. 80 °C)
Amersham Protran® supported Nitrocellulose electrostatic, hydrophobic unknown 86-110 0,2/0,45 UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores Size (kD)
genomic DNA (human) >11000000
E. coli approx. 1500000
0.45 μm approx. 5000
0.2 μm approx. 2000
DNA 3 kb approx. 1000
RNA 1 kb approx. 300
Antibodies (IgG) approx. 150
Proteins in the lab approx. 10–300
BSA (Albumin) 69

Application of ROTH-Transfer Membranes

Biomolecules Nylon plus Nylon 0.2 PVDF 0.2 PVDF Fluoro PVDF NC NC 0.45
DNA +++ + - - - + +
RNA + +++ - - - + +
Proteins - + +++ +++ +++ + +
Detection System
Colour +++ +++ +++ +++ +++ +++ +++
Radioactivity +++ +++ +++ +++ +++ +++ +++
Chemoluminescence +++ +++ +++ +++ - + +
Fluorescence +++ +++ - - +++ - -
+++: recommended +: suitable -: not