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ROTI®Cell DMEM High Glucose

CELLPURE® ready-to-use, sterile, with stable glutamine, with pyruvate
Icon_Steril Sterile products
Icon_ready-to-use Ready-to-use products and reagents
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Verp.
DMEM cell culture medium, Dulbecco’s Modified Eagle Medium
Density (D) ~1 g/cm³
Boiling point (bp) ~100 °C
Storage temp. +4 °C
Transport temp. ambient temp.
WGK 1

Medium for a wide range of mammalian cell culture applications.

18,85 €/VE 

Excl. btw | 500 ml Per VE

Bestelnr. 9007.1

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Productdetails

Steriel


ROTI®Cell DMEM High Glucose CELLPURE® ready-to-use, sterile, with stable glutamine, with pyruvate

Technische informatie
Toepassing Cell culture 
Gebruikstip Store at +2°C to +8°C protected from light. Once opened, store at 4°C and use within 6-8 weeks. 
Aanvullende informatie The detailed composition can be found in the specification. 
ROTI®Cell DMEM High Glucose
Geselecteerde hoeveelheid:   0
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Bestelnr. VE Verp. Prijs Hoeveelheid
9007.1 500 ml plastic

18,85 €

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In bestelling
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Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS


Algemene informatie

The buffer system in cell culture media

In addition to the various nutrients in the medium, the pH value also plays a very important role in cell culture. The physiological pH for almost all mammalian cells is 7.4. Deviating from this, it has been observed that certain transformed cell lines prefer a pH between 7.0 and 7.4 and certain fibroblasts prefer a pH between 7.4 and 7.7. In any case, pH fluctuations should be avoided and the pH should be maintained as well as possible. That is why a buffer system in media is necessary. The most important buffer system in the body is the carbonic acid-carbonate buffer system. Accordingly, almost all cell culture media contain bicarbonate (NaHCO3). Bicarbonate alone leads to a strong pH increase of the medium, so that NaHCO3-buffered media must always be used with CO2 incubators. Depending on the NaHCO3 concentration in the medium, different CO2 environments are necessary to ensure a pH of about 7.4. For this reason, you should not work outside of the CO2 incubator for too long. If this is necessary, a HEPES buffer should be used in addition to the NaHCO3, which contributes as a second buffer system to stabilize the pH value.


ROTI®Cell Media for Cell Culture - Composition

Medium Art. No. Glucose
g/l
Glutamine
(mM)
HEPES
(mM)
Sodium Pyruvate
(mM)
NaHCO3
(mM)
Phenol
Red
Salts Note
Dulbecco’s Modified Eagle Media
ROTI®Cell DMEM High Glucose 9005.1 4.5 4 X 1 44 yes X

ROTI®Cell DMEM High Glucose 9006.1 4.5 4 X X 44 yes X

ROTI®Cell DMEM High Glucose 9007.1 4.5 4 stab. X 1 44 yes X With temperature stabile glutamine.
ROTI®Cell DMEM High Glucose 1TE9.1 4,5 4 stab. 25 X 44 yes X With temperature stabile glutamine.
ROTI®Cell DMEM High Glucose 9010.1 4.5 X X 1 44 yes X

ROTI®Cell DMEM High Glucose 9019.1 4.5 X X X 44 yes X

ROTI®Cell DMEM High Glucose 1TE8.1 4.5 X X X 44 X X Without phenol red.
ROTI®Cell DMEM Low Glucose 1TE7.1 1.0 4 stab. X 1 44 yes

With temperature stabile glutamine.
ROTI®Cell DMEM Low Glucose 9027.1 1.0 X X 1 44 yes X

DMEM : F12
ROTI®Cell DMEM : F12 1TE5.1 3.151 2.5 15 1 14.3 yes X

Iscove’s Modfied Dulbecco Media
ROTI®Cell Iscove’s MDM 9033.1 4.5 4 25 1 36 yes X Additional amino acids and selene.
Minimum Essential Media
ROTI®Cell Eagle’s MEM / Earle’s 9044.1 1 2 X X 26.2 yes Earle’s

ROTI®Cell Eagle’s MEM / Earle’s 1TE6.1 1 2 stab. X X 26.2 yes Earle’s With temperature stabile glutamine.
ROTI®Cell Eagle’s MEM / Earle’s 9047.1 1 X X X 26.2 yes Earle’s

ROTI®Cell Eagle’s MEM-Alpha 9058.1 1 X X 1 26.2 yes Earle’s Plus cobalamin, ascorbic acid, NEAA*, lipoic acid, biotin.
ROTI®Cell Eagle’s MEM-Alpha 1TEA.1 1 X X 1 26.2 yes Earle’s Plus cobalamin, ascorbic acid, NEAA*, lipoic acid, biotin, ribonucleosides and desoxyribonucleosides.
RPMI Media
ROTI®Cell RPMI 1640 9085.1 2 2 X X 23.8 yes X

ROTI®Cell RPMI 1640 9086.1 2 2 25 X 23.8 yes X

ROTI®Cell RPMI 1640 9091.1 2 2 stab. X X 23.8 yes X With temperature stabile glutamine.
ROTI®Cell RPMI 1640 9099.1 2 X X X 23.8 yes X

ROTI®Cell RPMI 1640 9104.1 2 X X X 23.8 X X

Special Media
ROTI®Cell Ham’s F12 9108.1 1.8 1 X 1 14 yes X

ROTI®Cell Leibovitz’s L15 9109.1 0.9 galact. X X 5 X yes X With galactose instead of glucose. Without sodium bicarbonate.
ROTI®Cell McCoy’s 5A 9111.1 3 1.5 X X 26.2 yes X

ROTI®Cell Medium 199 / Earle’s 9112.1 1 0.7 X X 26.2 yes Earle’s

* NEAA: Non-essential amino acids

ROTI®Cell Media for Cell Culture - Application

Medium Art. No. CO2 (%) Serum Field of application Successfully cultured cells
Dulbecco’s Modified Eagle Media
ROTI®Cell DMEM 9005.1 8,5 recommend Frequently used universal medium with up to 4-fold higher concentrations of amino acids and vitamines. Primary fibroblasts, neurons, glial cells, smooth muscle cells, epithelial cells, tumor cells, hybridome culturesHUVEC, HeLa, HEK 293, PC-12NIH 3T3, 3T6, A-431, A9 L, BALB 3T3, BSC40, COS-1, COS-7, EB, L6, PK1
9006.1
9007.1
1TE9.1
9010.1
9019.1
1TE8.1
1TE7.1
9027.1
DMEM : F12
ROTI®Cell DMEM : F12 1TE5.1 5,5 optional Highly complex medium for versatile use.Basis of many serum-free media. Primary fibroblasts, neurons, HU-VECs, smooth muscle cellsHEK-293, HeLa, Cos-7, PC-12
Iscove’s Modfied Dulbecco Media
ROTI®Cell Iscove’s MDM 9033.1 7,0 optional For fast proliferating cells, high cell densities and mass cell cultures. Bone marrow cells, lymphocytes, macrophages, Hybridoma culturesKG-1, COS-7, Jurkat
Minimum Essential Media
ROTI®Cell Eagle’s MEM / Earle’s 9044.1 5,0 recommend For cells with special nutritionalrequirements. Numerous suspension and adherent cells, mammalian fibroblastsHek293, BS-C-1, CV-1, Hep G2, JEG-3, L-929, McCoy, MDBK, MDCK, PK(15), PtK1, RK13, Vero
ROTI®Cell Eagle’s MEM / Earle’s 1TE6.1 5,0 recommend
ROTI®Cell Eagle’s MEM / Earle’s 9047.1 5,0 recommend
ROTI®Cell Eagle’s MEM-Alpha 9058.1 5,0 recommend Additionally suitable for selection of transfected DG44 and other AHFR-negative cells. BHK-21, BT, Caco-2, CRFK, D-17, HeLa, HT-1080, XC
ROTI®Cell Eagle’s MEM-Alpha 1TEA.1 5,0 recommend For cells with special nutritionalrequirements.
RPMI Media
ROTI®Cell RPMI 1640 9085.1 4,5 recommend For single cell lines also suitable without serum. Lymphocytes, leukemia cells, hybridoma cultures, astrocytes, carcinoma cellsDaudi, H9, HCT-15, HL-60, IM-9, Jurkat, K-562, Raji, MCF-7, HeLa, PC12, PBMC
9086.1
9091.1
9099.1
9104.1
Special Media
ROTI®Cell Ham’s F12 9108.1 2,5 optional Widely used, highly complex medium. Specifically for mouse CHO cells, lung cells and L cellsCHO-K1
ROTI®Cell Leibovitz’s L15 9109.1 0,0 recommend Suitable for fast growing cells in relatively high density.Cultivation outside the CO2 incubator. Tumor and embryonic cellsHeLa, Hep-2, SW-13
ROTI®Cell McCoy’s 5A 9111.1 5,0 recommend Standard medium for clonal growth of hepatoma cell linesUsed in virus production. Hepatoma cell lines, other permanent cell lines and also primary cellsHT-29, Jensen
ROTI®Cell Medium 199 / Earle’s 9112.1 5,0 optional Highly complex medium versatile. Especially suitable for non-transformed cellsLLC-PK1, LLC-WRC 256

Analysecertificaten

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De volgende analysecertificaten werden gevonden:

Type analysis

Glucose4,5 g/l
L-Glutamine, stablecomplies
pH value7.0-7,6
Osmolality325-360 mOsm/kg
Endotoxins≤1,0 EU/ml
Sterilitycomplies