product.details.print
Transfer membrane Amersham™ Protran® NC 0.45 roll 400 × 20 cm
WGK 1
Replaces Protran® BA 85 from Whatman®
Multiple use - ideal for most nucleic acid and protein immobilising techniques. Pure nitro-cellulose, free from cellulose acetate and triton. They have a high binding capacity and low background.
Bestelnr. 0041.1
Geschikt voor / Toebehoren
Productdetails
Transfer membrane for most nucleic acid and protein immobilising techniques
Replaces Protran® BA 85 from Whatman®
Multiple use - ideal for most nucleic acid and protein immobilising techniques. Pure nitro-cellulose, free from cellulose acetate and triton. They have a high binding capacity and low background.
Nitrocellulose should only conditionally be used for electrophoretic transfer of nucleic acids, because of the high salt concentrations required for the transfer.
Membranes of 0,45 μm pore size allow for DNA transfer (minimum 300 bp) and protein transfer (minimum 20 kDa).
Southern, Northern, Western transfer, colony and plaque hybridisation, immunoblots, nucleic acid and protein dot-blot, slot-blot, electrophoretic transfer of proteins.
Detection systems are radioactivity, chemoluminescence, colour
Transfer membrane Amersham™ Protran® NC 0.45 roll 400 × 20 cm
In practical roll format for maximum flexibility in cutting.
| Afmeting | 400 × 20 cm |
| Uitvoering | roll |
| Poriëngr. | 0,45 µm |
| Type | Protran® NC 0,45 |
| Binding | electrostatic, hydrophobic |
| Immobilisering | UV, baking (max. 80 °C) |
| Bindingscapaciteit | 86-110 µg/cm² |
- Tussentotaal: 0.00
| Bestelnr. | Afmeting | VE | Prijs | Hoeveelheid | |
|---|---|---|---|---|---|
| 0041.1 | 400 × 20 cm | 1 rol(len) | NV |
|
|
|
Op voorraad
Beschikbaar (let op levertijd)
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
|
|||||
- Tussentotaal: 0.00
Downloads / MSDS
Algemene informatie
Technical Parameters of ROTH-Transfer Membranes
| Membrane | Material | Characteristics | Thickness (μm) | Binding capacity (μg/cm2) | Pore size (μm) | Immobilisation |
| Roti® Fluoro PVDF | Polyvinyliden fluoride | hydrophobic | 125 ±25 | 300 | 0,2 | not necessary |
| Transfer membrane ROTI®PVDF 0.2 | Polyvinyliden fluoride | hydrophobic | 190 ±50 | ≥125 | 0,2 | not necessary |
| Transfer membrane ROTI®PVDF 0.45 | Polyvinyliden fluoride | hydrophobic | 190 ±50 | ≥125 | 0,45 | not necessary |
| Immobilon P | Polyvinyliden fluoride | hydrophobic | 120 ±25 | 150-300 | 0,45 | not necessary |
| Roti® Nylon 0.2 | Nylon 6,6 | neutral/amphoteric | 150 ±20 | 500 | 0,2/0,45 | UV, baking |
| Roti® Nylon plus | Nylon 6,6 | hydrophilic, positively charged | 160 ±20 | 500 | 0,45 | not necessary, UV and baking possible |
| Transfer membrane ROTI®NC 0.2 | Nitrocellulose | hydrophobic | 150 ±50 | 100 | 0,2 | UV, baking (max. 80 °C) |
| Transfer membrane ROTI®NC 0.45 | Nitrocellulose | hydrophobic | 150 ±50 | 100 | 0,45 | UV, baking (max. 80 °C) |
| Amersham Protran® | Nitrocellulose | electrostatic, hydrophobic | unknown | 11-72 | 0,2/0,45 | UV, baking (max. 80 °C) |
| Amersham Protran® supported | Nitrocellulose | electrostatic, hydrophobic | unknown | 86-110 | 0,2/0,45 | UV, baking (max. 80 °C) |
| Biomolecules / Membrane Pores | Size (kD) |
| genomic DNA (human) | >11000000 |
| E. coli | approx. 1500000 |
| 0.45 μm | approx. 5000 |
| 0.2 μm | approx. 2000 |
| DNA 3 kb | approx. 1000 |
| RNA 1 kb | approx. 300 |
| Antibodies (IgG) | approx. 150 |
| Proteins in the lab | approx. 10–300 |
| BSA (Albumin) | 69 |
| Biomolecules | Nylon plus | Nylon 0.2 | PVDF 0.2 | PVDF | Fluoro PVDF | NC | NC 0.45 |
| DNA | +++ | + | - | - | - | + | + |
| RNA | + | +++ | - | - | - | + | + |
| Proteins | - | + | +++ | +++ | +++ | + | + |
| Detection System | |||||||
| Colour | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Radioactivity | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Chemoluminescence | +++ | +++ | +++ | +++ | - | + | + |
| Fluorescence | +++ | +++ | - | - | +++ | - | - |
| +++: recommended | +: suitable | -: not | |||||