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Transfer membrane ROTI®NC 0.2, 300 × 30 cm
High-quality transfer membrane for all detection systems - extremely suitable for all protein transfer and detection methods. The membrane stands out for its particularly high mechanical stability. The pure 100 % nitrocellulose quality membrane is easy to block and allows for background-free blots with perfect bands.
405,30 €/VE
Excl. btw | 1 rol(len) Per VE
Bestelnr. 1YXN.1
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Productdetails
Transfer membrane for protein analyses, Southern- and Northern-Blots.
High-quality transfer membrane for all detection systems - extremely suitable for all protein transfer and detection methods. The membrane stands out for its particularly high mechanical stability. The pure 100 % nitrocellulose quality membrane is easy to block and allows for background-free blots with perfect bands.
Transfer membrane ROTI®NC 0.2
The pore size of 0,2 μm is suitable for proteins and peptides of all sizes. The best retention rate is achieved when blotting proteins of up to 70 kDa. Its excellent protein binding capacity not only efficiently prevents the blot from going through during transfer, but also guarantees highly sensitive detection of even low protein amounts.
A slight optical difference between the two membrane sides results from the membrane production and has no influence on the binding capacity or the blot quality of the two sides. Both sides can be used equally for transfer and detection.We recommend to always use the optically same side as “detection side” to achieve maximum reproducibility.
Optimised for proteins of ≤70 kDa. Western- and far-western-blot, colony- and plaque-lifting of nucleic acids and proteins, immunoblots, dot-blots.
Detection via radio activity, chemoluminescence colour.
- Western Blotting
- Protein and Immunoblotting
- Northern Blotting
- Southern Blotting
- Dot / Slot Blotting
| Afmeting | 300 × 30 cm |
| Uitvoering | roll |
| Poriëngr. | 0,2 µm |
| Binding | hydrophobic |
| Dikte | 150±50 µm |
| Immobilisering | UV, baking (max. 80 °C) |
| Bindingscapaciteit | 100 µg/cm² |
- Tussentotaal: 0.00
| Bestelnr. | Afmeting | VE | Prijs | Hoeveelheid | |
|---|---|---|---|---|---|
| 1YXN.1 | 300 × 30 cm | 1 rol(len) |
405,30 € |
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| 200E.1 | 300 × 20 cm | 1 rol(len) |
277,35 € |
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| 200H.1 | 20 × 20 cm | 5 blad |
135,65 € |
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| 204H.1 | 10 × 10 cm | 10 blad |
124,25 € |
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- Tussentotaal: 0.00
Downloads / MSDS
Algemene informatie
Technical Parameters of ROTH-Transfer Membranes
| Membrane | Material | Characteristics | Thickness (μm) | Binding capacity (μg/cm2) | Pore size (μm) | Immobilisation |
| Roti® Fluoro PVDF | Polyvinyliden fluoride | hydrophobic | 125 ±25 | 300 | 0,2 | not necessary |
| Transfer membrane ROTI®PVDF 0.2 | Polyvinyliden fluoride | hydrophobic | 190 ±50 | ≥125 | 0,2 | not necessary |
| Transfer membrane ROTI®PVDF 0.45 | Polyvinyliden fluoride | hydrophobic | 190 ±50 | ≥125 | 0,45 | not necessary |
| Immobilon P | Polyvinyliden fluoride | hydrophobic | 120 ±25 | 150-300 | 0,45 | not necessary |
| Roti® Nylon 0.2 | Nylon 6,6 | neutral/amphoteric | 150 ±20 | 500 | 0,2/0,45 | UV, baking |
| Roti® Nylon plus | Nylon 6,6 | hydrophilic, positively charged | 160 ±20 | 500 | 0,45 | not necessary, UV and baking possible |
| Transfer membrane ROTI®NC 0.2 | Nitrocellulose | hydrophobic | 150 ±50 | 100 | 0,2 | UV, baking (max. 80 °C) |
| Transfer membrane ROTI®NC 0.45 | Nitrocellulose | hydrophobic | 150 ±50 | 100 | 0,45 | UV, baking (max. 80 °C) |
| Amersham Protran® | Nitrocellulose | electrostatic, hydrophobic | unknown | 11-72 | 0,2/0,45 | UV, baking (max. 80 °C) |
| Amersham Protran® supported | Nitrocellulose | electrostatic, hydrophobic | unknown | 86-110 | 0,2/0,45 | UV, baking (max. 80 °C) |
| Biomolecules / Membrane Pores | Size (kD) |
| genomic DNA (human) | >11000000 |
| E. coli | approx. 1500000 |
| 0.45 μm | approx. 5000 |
| 0.2 μm | approx. 2000 |
| DNA 3 kb | approx. 1000 |
| RNA 1 kb | approx. 300 |
| Antibodies (IgG) | approx. 150 |
| Proteins in the lab | approx. 10–300 |
| BSA (Albumin) | 69 |
| Biomolecules | Nylon plus | Nylon 0.2 | PVDF 0.2 | PVDF | Fluoro PVDF | NC | NC 0.45 |
| DNA | +++ | + | - | - | - | + | + |
| RNA | + | +++ | - | - | - | + | + |
| Proteins | - | + | +++ | +++ | +++ | + | + |
| Detection System | |||||||
| Colour | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Radioactivity | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Chemoluminescence | +++ | +++ | +++ | +++ | - | + | + |
| Fluorescence | +++ | +++ | - | - | +++ | - | - |
| +++: recommended | +: suitable | -: not | |||||