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Transfer membrane ROTI®Nylon 0.45, 300 × 30 cm
All autoclavable products in our catalogue are identified by this symbol. Please also note any additional instructions when autoclaving.
456,90 €/VE
Excl. btw | 1 rol(len) Per VE
Bestelnr. 260P.1
Geschikt voor / Toebehoren
Productdetails
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Transfer membrane ROTI®Nylon 0.45
The optimal membrane for DNA/RNA transfer, especially colony and plaque lifting and all multiple rehybridisations.
ROTI®Nylon 0.45 is a neutral, reinforced nylon membrane. Like all ROTI®Nylon membranes, it has a very high tensile strength and is highly resistant to alcohols, acetone and many other organic solvents.
ROTI®Nylon 0.45 is not charged at buffer pH 6.5 and has excellent hydrophilic properties due to the amino and carboxy groups on the surface. The membrane charge (ζ-potential) can be adjusted by the pH value of the buffer system (positive membrane charge at pH<6.5; negative membrane charge at pH>6.5). Steam sterilisation is possible.
The covalent binding of transferred nucleic acid is achieved by crosslinking (UV crosslinking for 20 s at 260 nm). The excellent signal/background ratio of the membrane then enables highly sensitive detections (single copy gene detection) and multiple rehybridisations without loss of signal quality.
Detection by radioactivity, chemiluminescence, fluorescence
- Southern and Northern blotting
- Western blotting
- Microarrays and Macroarrays
- Dot/Slot blotting
| Afmeting | 300 × 30 cm |
| Poriëngr. | 0,45 µm |
| Binding | hydrophile |
| Lading | neutral/amphoteric |
| Dikte | 140-190 µm |
| Immobilisering | UV |
| Bindingscapaciteit | 350 µg/cm² |
- Tussentotaal: 0.00
| Bestelnr. | Afmeting | VE | Prijs | Hoeveelheid | |
|---|---|---|---|---|---|
| 260P.1 | 300 × 30 cm | 1 rol(len) |
456,90 € |
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| 260T.1 | 300 × 15 cm | 1 rol(len) |
236,50 € |
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| 260X.1 | 20 × 20 cm | 5 blad |
166,65 € |
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| 260Y.1 | 15 × 15 cm | 5 blad |
118,25 € |
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- Tussentotaal: 0.00