ROTIPHORESE®NF-Acrylamide/Bis-solution 40 (19:1)
Boiling point (bp) ~100 °C
Storage temp. +4 °C
ADR 6.1 III
Optimal for DNA gels for separation of small fragments, manual sequencing and automatic sequencers. Cross linking 5.
Excl. btw | 250 ml Per VE
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Ready-to-use, gas-stabilized ready-to-use acrylamide solutions with different concentrations and ratios of acrylamide to bisacrylamide. The ROTIPHORESE® gel solutions are very easy to use and enable reproducible and high-resolution gel electrophoresis.
ROTIPHORESE® gel solutions can be used for protein and nucleic acid separation in PAGE gels. The high-purity solutions can be combined with any buffer system. During sequencing, they facilitate the preparation of sequencing gels of consistent quality; our fluorescence-free gel solutions are recommended for the use of automatic sequencers.
The ROTIPHORESE®acrylamide and bisacrylamide solutions from Carl ROTH are produced only from acrylic acid-free substances and are strictly controlled in acrylamide content and pH. Oxidation products are removed from the solution by a selective absorption process. Their new formation and the polymerization of the solution are prevented by stabilizing the solution with inert gas.
In order to ensure a high lot-to-lot consistency of our ROTIPHORESE® solutions, each batch is subjected to an electrophoretic test. Fluorescence-free gel solutions are tested for suitability for automatic sequencers.
ROTIPHORESE®NF ready-to-use solutions for automated sequencing are special fluorescence-free acrylamide/bisacrylamide mixtures of highest quality as required for automatic sequencing. Made from 4x cristallised acrylamide.
• For preparing 4-20 % sequencing gels
• No tedious dissolving of urea
• Prevents acrylamide dusts
• Simple handling
NF acrylamide solutions are very well suited for application in fluorescence-labelled automated sequencing, e.g. using LI-COR or ABI slab gel sequencers.
ROTIPHORESE®NF-Acrylamide/Bis-solution 40 (19:1) ROTIPHORESE® 40 %, ready-to-use, gas-stabilized, fluorescence-free
Cross linking 5.
ROTIPHORESE®NF ready-to-use solutions are made from 4x crystallized acrylamide and are special fluorescence-free acrylamide/bisacrylamide mixtures of the highest quality, as required for automatic sequencing.
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Acrylamide ia a strong nerve poison which has clearly proved to be carcinogenic and to change the genetic make-up during animal testing. Acrylamide is resorbed primarily through the skin. The formation of dust when working with the solid matter is particularly critical. A hazard which can be easily avoided by using the ROTIPHORESE® ready-to-use solution.
ROTIPHORESE® Acrylamide and -Bisacrylamide solutions from Carl ROTH have the highest purity level. They are produced from substances free from acrylic acid. Oxidation products are removed from the solution by a selective absorption process. A new formation of such and/or polymerisation of the solution is prevented by gaseous stabilisation of the solution.
In order to guarantee a high consistency of our ROTIPHORESE®-solutions, each batch undergoes electrophoretic testing.
The right choice for each and every application
The large variety of different ROTIPHORESE® solutions provides you with the right product for each and every application. ROTIPHORESE® gel solution may be used both in protein and nucleic acid electrophoresis. ROTIPHORESE® ready-to-use solutions for manual sequencing facilitate the preparation of sequencing gels of a constant high quality - also suitable for automatic sequencers.
ROTIPHORESE®Gel 30 and Gel 40 are ready-to-use, gas-stabilised acrylamide solutions with different concentrations and with different ratios of acrylamide to bisacrylamide. ROTIPHORESE® ready-to-use solutions can be used directly.
Recommendation for Use of ROTIPHORESE® Ready-Made Gel Solutions
|Analysis of||Gel solution||Art. No.|
|Proteins/peptides >15 kDa, DNA fragments||Gel 30 (37,5:1)||3029.1|
|Gel 40 (37,5:1)||T802.1|
|Proteins/peptides <30 kDa and DNA oligonucleotides||Gel 40 (29:1)||A515.1|
|DNA sequences (manual sequencing)||ROTIPHORESE®DNA sequencing system||A431.1|
|Gel 40 (19:1)||3030.1|
|DNA sequences (short-run automat. sequencing)||NF solution 40 % (19:1)||A516.1|
|DNA sequences (long-run automat. sequencing)||NF solution 30 % (29:1)||A124.1|
|NF solution 40 % (29:1)||A121.1|
|Filled under protective gas., Tested for automatic sequencers., Each batch is functionally tested for its suitability in electrophoresis.|