ROTIPHORESE®PAGE Matrix Buffer plus, 250 ml, 1 x 250 ml
Transport temp. cooled
One bottle of 250 ml is sufficient for pouring of approx. 100 mini gels.
Excl. btw | 250 ml Per VE
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- Increases protein separation quality significantly
- Superior band dissemination from 5 kDa to 250 kDa
- Higher gel strength and longer shelf life
- Compatible with all common gel loading and running buffers
- Compatible with all common gel staining methods
- Also well suitable for 2D gels and prior to Western Blotting
- 250 ml sufficient for approx. 100 mini gels
ROTIPHORESE®PAGE Matrix Buffer plus is a 4x concentrated gel matrix buffer for enhanced protein separation and significantly higher gel strenth.
ROTIPHORESE®PAGE Matrix Buffer plus can be used for preparation of both stacking gel and resolving gel by simply replacing the Laemmli/TRIS-, or TRICIN-, buffer system. Even gels with stacking gels of only one acrylamide concentration, show a protein separation quality comparable to that of gradient gels. Band depiction is significantly enhanced compared to standard buffer systems.
ROTIPHORESE®PAGE Matrix Buffer plus also provides increased gel strength, making handling of the gels much more convenient, for instance during separation of the glass plates or during transport. Gel stability is increased, leading to longer shelf lifes of self-made gels. Gels prepared with ROTIPHORESE®PAGE Matrix Buffer plus may also very well be used for 2D protein analysis or for subsequent Western Blotting onto NC or PVDF membranes.
ROTIPHORESE®PAGE Matrix Buffer plus ROTIPHORESE® 4x conc., for electrophoresis
Stability of a 6 % PA gel.
Coomassie® stained gel and Western blot. HeLa extract on PVDF, detected via HRP chemoluminescence.
A: 10 % PAGE using ROTIPHORESE®PAGE Matrix buffer plus,
B: 12 % PAGE using standard Tris glycine SDS buffer.
Silver staining. HL-60 cell extract after 1D-IEF (pH 4-7) and 2D gel electrophoresis in a 12 % PA gel using ROTIPHORESE®PAGE Matrix buffer plus.
- Tussentotaal: 0.00
|8996.1||250 ml||plastic||1 x 250 ml||
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Acrylamide ia a strong nerve poison which has clearly proved to be carcinogenic and to change the genetic make-up during animal testing. Acrylamide is resorbed primarily through the skin. The formation of dust when working with the solid matter is particularly critical. A hazard which can be easily avoided by using the ROTIPHORESE® ready-to-use solution.
ROTIPHORESE® Acrylamide and -Bisacrylamide solutions from Carl ROTH have the highest purity level. They are produced from substances free from acrylic acid. Oxidation products are removed from the solution by a selective absorption process. A new formation of such and/or polymerisation of the solution is prevented by gaseous stabilisation of the solution.
In order to guarantee a high consistency of our ROTIPHORESE®-solutions, each batch undergoes electrophoretic testing.
|Remark: The product may crystallise. Can be redissolved by heating (max. 40 °C).|