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1NNP 1NNA_01 1NNA_02 1NNP_offen

anti-6-His-tag mouse monoclonal, 6His, 25 µg
Van  PROGEN
lyophilized, monoclonal, affinity purified
Icon_Abweichende-Konditionen
Verwante producten
VE
Verp.
Primary antibodies
Storage temp. +4 °C
Transport temp. ambient temp.

Binding of His-tag proteins
0.25 mg/ml after reconstitution with 100 μl / 400 μl PBS

Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.
Productdetails

117,20 €/VE 

Excl. btw | 25 µg Per VE

Bestelnr. 1NNA.1

Beschikbaar
Vrij van verzendingskosten vanaf 250 €
Klaar voor verzending in 1-2 weken
Vanaf 6 VE 111,34 €/VE
Vanaf 24 VE 105,48 €/VE

Geschikt voor / Toebehoren

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Productdetails


Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.

  • Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep)
  • High affinity to the respective protein tag
  • Robust & highly specific detection of the fusion protein
  • Unconjugated
  • Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence


anti-6-His-tag mouse monoclonal, 6His lyophilized, monoclonal, affinity purified

The monoclonal 6-His antibody recognizes polyhistidine (6-His). The 6-His tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.

Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: 6His
Immunogen: recombinant protein containing the sequence HHHHHH
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, IP, WB
Reactivity: 6-His


Toepassing

Immunocytochemistry (ICC): assay dependent
Immunoprecipitation (IP): assay dependent
Western Blot (WB): 1:2,000-1:5,000 (0.125-0.05 μg/ml)


1NNA_01
Western blot analysis of E. coli lysate containing 6-His-tagged protein with anti-6-His-tag antibody. Western blot analysis was performed on 160 ng, 80 ng or 20 ng of E. coli lysate containing 6-His-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-6-His-tag mouse monoclonal, 6His (Cat. No. 910HISL) was diluted in blocking buffer (antibody concentration 0.125 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
1NNA_02
Western blot analysis of E. coli lysate containing 6-His-tagged protein with anti-6-His-tag antibody. Western blot analysis was performed on 50 ng or 25 ng of E. coli lysate containing 6-His-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-6-His-tag mouse monoclonal, 6His (Cat. No. 910HISL) was diluted in blocking buffer (antibody concentration 0.05 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.


Geen medisch hulpmiddel / Geen ivD-product
anti-6-His-tag mouse monoclonal, 6His
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Prijs Hoeveelheid
1NNA.1 25 µg plastic

117,20 €

1NNA.2 100 µg plastic

424,65 €

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Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS

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