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anti-Cardiac Actin mouse monoclonal, AC1-20.4.2, 0.2 ml

Van  PROGEN
50 μg/ml, monoclonal, affinity purified
Icon_Abweichende-Konditionen Bitte beachten: Abweichende Konditionen / Please note: Different conditions
Verwante producten
VE
Verp.
Antibody, Primary antibodies
Density (D) ~1 g/cm³
Boiling point (bp) ~100 °C
Storage temp. +4 °C
Transport temp. ambient temp.

Cytoskeleton marker

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
Productdetails

103,10 €/VE 

Excl. btw | 0,2 ml Per VE

Bestelnr. 1NLN.1

Beschikbaar
Vrij van verzendingskosten vanaf 250 €
Klaar voor verzending in 1-2 weken
Vanaf 6 VE 97,94 €/VE
Vanaf 24 VE 92,79 €/VE

Productdetails


This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

  • High epitope affinity
  • Highly specific detection of the target protein
  • Unconjugated
  • Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence


anti-Cardiac Actin mouse monoclonal, AC1-20.4.2 50 μg/ml, monoclonal, affinity purified

Ac1 represents an excellent marker for cardiac tissue; it discriminates fetal (cardiac) a-actin from all other actin isoforms. Fetal actin can be localized in regenerating skeletal muscle after injury (in satellite cells) and in veins of the umbelical cord. Mab Ac1-20.4.2 shows no cross reaction with other actin isoforms present in skeletal and smooth muscle, provided that stringent experimental conditions have been applied.

Polypeptide reacting: specific for fetal (cardiac) isoform of actin.

Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: AC1-20.4.2
Immunogen: synthetic NH2 terminus decapeptide of cardiac isoform of actin
UniprotID: P68034 (chicken), P68032 (human), G1STB6 (rabbit)
Synonym: actin, alpha cardiac muscle 1, alpha-cardiac actin [cleaved into: actin, alpha cardiac muscle 1, intermediate form], ACTC1, ACTC
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: IHC, WB
Reactivity: bovine, chicken, human, mouse, rabbit


Toepassing

Immunohistochemistry (IHC) - frozen: 1:10 (5 μg/ml; include 0.5 M NaCl in all washing buffers to enhance specificity)
Immunohistochemistry (IHC) - paraffin: 1:10 (5 μg/ml; microwave treatment recommended)
Western Blot (WB): 1:1,000 (50 ng/ml; include 1 M NaCl in all washing buffers to enhance specificity)


1NLN_01
Western blot analysis of human heart whole tissue lysate and with anti-Cardiac Actin antibody. Western blot analysis was performed on 20 μg human heart lysate. The PVDF membrane was blocked with 5% milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Cardiac Actin mouse monoclonal, AC1-20.4.2 (Cat. No. 690075) was diluted in blocking buffer (antibody concentration 0.05 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
1NLN_02
anti-Cardiac Actin mouse monoclonal, AC1-20.4.2, liquid, purified


Geen medisch hulpmiddel / Geen ivD-product
anti-Cardiac Actin mouse monoclonal, AC1-20.4.2
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Prijs Hoeveelheid
1NLN.1 0,2 ml plastic

103,10 €

1NLN.2 1 ml plastic

267,70 €

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Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

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Analysecertificaten

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De volgende analysecertificaten werden gevonden:

Type analysis

ClonalityMonoclonal
Concentration50 µg/ml
Raised inHybridoma cell culture supernatant
Immunglobulin (Ig) classIgG1
CloneAC1-20.4.2
ConjugateUnconjugated
PurificationAffinity chromatography
Purity (SDS-PAGE)≥95 %
PBS buffer, pH 7.4 with 0.09 % sodium azide and 0.5 % BSA., Short term at 2-8 °C (+4 °C); long term storage in aliquots at -20 °C; avoid freeze/thaw cycles.