anti-DDDDK-tag mouse monoclonal, AP1501, 25 µg | Primary Antibodies | Antibodies | Western & ELISA | Protein Biology | Life Science

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Analysecertificaten

anti-DDDDK-tag mouse monoclonal, AP1501, 25 µg

Van PROGEN

lyophilized, monoclonal, affinity purified

Downloads / MSDS

Analysecertificaten

Bitte beachten: Abweichende Konditionen / Please note: Different conditions

Verwante producten

Verp.

Primary antibodies

Storage temp. +4 °C
Transport temp. ambient temp.

0.25 mg/ml after reconstitution with 100 μl / 400 μl PBS
Binding of DDDDK-tag proteins

Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.

Productdetails

117,20 €/VE

Excl. btw | 25 µg Per VE

Bestelnr. 1NLE.1

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Vrij van verzendingskosten vanaf 250 €

Klaar voor verzending in 1-2 weken

Vanaf 6 VE 111,34 €/VE

Vanaf 24 VE 105,48 €/VE

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Productdetails

Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep)
High affinity to the respective protein tag
Robust & highly specific detection of the fusion protein
Unconjugated
Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

anti-DDDDK-tag mouse monoclonal, AP1501 lyophilized, monoclonal, affinity purified

The monoclonal AP1501 antibody recognizes the amino acid sequence DDDDK (Flag). The DDDDK-tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.

Host: mouse
Antibody type: monoclonal
Isotype: IgG2b
Clone: AP1501
Immunogen: recombinant protein containing the sequence DDDDK
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, IP, WB
Reactivity: DDDDK

Toepassing

Immunocytochemistry (ICC): assay dependent
Immunoprecipitation (IP): assay dependent
Western Blot (WB): 1:50,000-1:100,000 (0.005-0.0025 μg/ml)

Western blot analysis of E. coli lysate containing DDDDK-tagged protein with anti-DDDDK-tag antibody. Western blot analysis was performed on 80 ng, 40 ng or 10 ng of E. coli lysate containing DDDDK-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5 % dry milk in PBST for 1 h at RT. The primary antibody anti-DDDDK-tag mouse monoclonal, AP1501 (Cat. No. 910DDKL) was diluted in blocking buffer (antibody concentration 0.005 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

Geen medisch hulpmiddel / Geen ivD-product

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anti-DDDDK-tag mouse monoclonal, AP1501

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	Bestelnr.	VE	Verp.	Prijs
	1NLE.1	25 µg	plastic	117,20 € 117,20 €
	1NLE.2	100 µg	plastic	424,65 € 424,65 €
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