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anti-EP-CAM mouse monoclonal, HEA125, 1 ml

Van  PROGEN
50 μg/ml, monoclonal, affinity purified
Icon_Abweichende-Konditionen Bitte beachten: Abweichende Konditionen / Please note: Different conditions
Verwante producten
VE
Verp.
Antibody, Primary antibodies
Density (D) ~1 g/cm³
Boiling point (bp) ~100 °C
Storage temp. +4 °C
Transport temp. ambient temp.

Epithelium marker

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
Productdetails

267,70 €/VE 

Excl. btw | 1 ml Per VE

Bestelnr. 1NP1.2

Beschikbaar
Vrij van verzendingskosten vanaf 250 €
Klaar voor verzending in 1-2 weken
Vanaf 6 VE 254,32 €/VE
Vanaf 24 VE 240,93 €/VE

Productdetails


This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

  • High epitope affinity
  • Highly specific detection of the target protein
  • Unconjugated
  • Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence


anti-EP-CAM mouse monoclonal, HEA125 50 μg/ml, monoclonal, affinity purified

The Ep-CAM (HEA125) antibody recognizes the epithelial cell adhesion molecule Ep-CAM (also described as 17-1A antigen, EPG34, CD326, and TACSTD1). This antigen is widely expressed on cells of epithelial origin and tumors derived therefrom. HEA125 represents an excellent marker to discriminate epithelial from mesothelial structures. The antigen has been detected in all carcinoma types tested (18 different origins). A subset of squamous cell carcinoma is negative. The antibody can be used as alternative to the anti-EpCAM antibody clone BerEp4.

Polypeptide reacting: Mr 40,000 human epithelium-specific cell surface glycoprotein (Ep-CAM).

Reactivity on cultured cell lines: all carcinoma cell lines tested so far; particularly strong reaction with colon carcinoma cell lines.

Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: HEA125
Immunogen: HT-29 colon carcinoma cell line
Synonym: human epithelium antigen, HEA, 17-1A, EPG34, CD326, TACSTD1
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, IHC, WB
Reactivity: human
No reactivity: mouse


Toepassing

Immunocytochemistry (ICC): assay dependent
Immunohistochemistry (IHC) - frozen: 1:10-1:50 (1-5 μg/ml)
Immunohistochemistry (IHC) - paraffin: 1:10-1:50 (1-5 μg/ml; protease treatment recommended)
Western Blot (WB): 1:200-1:3.000 (0.02-0.25 μg/ml)


1NP1_01
Human head and neck squamous-cell carcinoma (HNSCC)(courtesy of J. Heß, University Hospital Heidelberg)
1NP1_02
Mouse stomach (courtesy of J.Heß, University Hospital Heidelberg)
1NP1_04
Western blot analysis of human HT29 cell lysate with anti-Ep-CAM antibody. Western blot analysis was performed on 20 μg of HT29 lysate. Cells were lysed with RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-EP-CAM mouse monoclonal, HEA125 (Cat. No. 690004) was diluted in blocking buffer (antibody concentration 0.025 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goat polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.


Geen medisch hulpmiddel / Geen ivD-product
anti-EP-CAM mouse monoclonal, HEA125
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Prijs Hoeveelheid
1NP1.1 0,2 ml plastic

103,10 €

1NP1.2 1 ml plastic

267,70 €

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Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

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