anti-GFP-tag mouse monoclonal, F56-6A1.2.3, 100 µg | Primary Antibodies | Antibodies | Western & ELISA | Protein Biology | Life Science

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Downloads / MSDS

Analysecertificaten

anti-GFP-tag mouse monoclonal, F56-6A1.2.3, 100 µg

Van PROGEN

lyophilized, monoclonal, affinity purified

Downloads / MSDS

Analysecertificaten

Bitte beachten: Abweichende Konditionen / Please note: Different conditions

Verwante producten

Verp.

Primary antibodies

Density (D) ~1 g/cm³
Boiling point (bp) 100 °C
Storage temp. +4 °C
Transport temp. ambient temp.

0.25 mg/ml after reconstitution with 100 μl / 400 μl PBS
Binding of GFP-tag proteins

Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.

Productdetails

435,40 €/VE

Excl. btw | 100 µg Per VE

Bestelnr. 1NP5.2

Beschikbaar (let op levertijd)

Klaar voor verzending in 1-2 weken

Vrij van verzendingskosten vanaf 250 €

Vanaf 6 VE 413,63 €/VE

Vanaf 24 VE 391,86 €/VE

In het winkelmandje

Geschikt voor / Toebehoren

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Productdetails

Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep)
High affinity to the respective protein tag
Robust & highly specific detection of the fusion protein
Unconjugated
Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

anti-GFP-tag mouse monoclonal, F56-6A1.2.3 lyophilized, monoclonal, affinity purified

The monoclonal F56-6A1.2.3 antibody recognizes the GFP-tag (green fluorescent tag). The GFP-tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.

Toepassing

Immunocytochemistry (ICC): assay dependent
Western Blot (WB): 1:500 (0.5 μg/ml)

Western blot analysis of E. coli lysate containing GFP-tagged protein with anti-GFP-tag antibody. Western blot analysis was performed on 250 ng, 100 ng or 25 ng of E. coli lysate containing GFP-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-GFP-tag mouse monoclonal, F56-6A1.2.3 (Cat. No. 910GFPL) was diluted in blocking buffer (antibody concentration 0.5 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

Geen medisch hulpmiddel / Geen ivD-product

Technische informatie

Gastheersoort	muis
Klonaliteit	monoclonal
Gastheersoort	IgG2b
Gastheersoort	Affinity chromatography
Gastheersoort	uncojugated
Referentiebanden	GFP-Tag
Referentiebanden	F56-6A1.2.3

Bestelformulier

In het winkelmandje

anti-GFP-tag mouse monoclonal, F56-6A1.2.3

Geselecteerde hoeveelheid: 0

In het winkelmandje

Tussentotaal: 0.00

	Bestelnr.	VE	Verp.	Prijs
	1NP5.1	25 µg	plastic	120,95 € 120,95 €
	1NP5.2	100 µg	plastic	435,40 € 435,40 €
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