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anti-Keratin K18 mouse monoclonal, Ks18.04, 0.2 ml

Van  PROGEN
50 μg/ml, monoclonal, affinity purified
Icon_Abweichende-Konditionen Bitte beachten: Abweichende Konditionen / Please note: Different conditions
Verwante producten
VE
Verp.
Antibody, Primary antibodies
Density (D) ~1 g/cm³
Boiling point (bp) ~100 °C
Storage temp. +4 °C
Transport temp. ambient temp.

Epithelium marker

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
Productdetails

103,10 €/VE 

Excl. btw | 0,2 ml Per VE

Bestelnr. 1NLY.1

Beschikbaar
Vrij van verzendingskosten vanaf 250 €
Klaar voor verzending in 1-2 weken
Vanaf 6 VE 97,94 €/VE
Vanaf 24 VE 92,79 €/VE

Productdetails


This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

  • High epitope affinity
  • Highly specific detection of the target protein
  • Unconjugated
  • Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence


anti-Keratin K18 mouse monoclonal, Ks18.04 50 μg/ml, monoclonal, affinity purified

Ks18.04 represents an excellent marker to discriminate simple epithelia from those of different origin.

Tumors specifically detected: all adenocarcinoma; mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma.

Polypeptide reacting: Mr 45,000 polypeptide (human keratin K18; formerly also designated cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia.

Tested cultured cell lines: MCF-7.

Host: mouse
Antibody type: monoclonal
Isotype: IgG1
Clone: Ks18.04
Immunogen: human keratin K18 from HeLa cytoskeletal preparation
UniprotID: A1XEA5 (Bovine),P05783 (human), P05784 (mouse), F1SGG1 (Pig),Q5BJY9 (rat), W5Q5M3 (sheep), W5Q5M3 (sheep)
Synonym: keratin, type I cytoskeletal 18, cell proliferation-inducing gene 46 protein, cytokeratin-18, CK-18, keratin-18, K18, KRT18, CYK18, PIG46
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: ICC/IF, IHC, WB
Reactivity: bovine, dog, hamster, human, mouse, pig, rat, sheep, trout, zebrafish


Toepassing

Immunocytochemistry (ICC): assay dependent
Immunohistochemistry (IHC) - frozen: 1:20-1:200
Immunohistochemistry (IHC) - paraffin: 1:20-1:200 (microwave treatment recommended)
Western Blot (WB): 1:100-1:500 (0.5 μg/ml-0.1 μg/ml)


1NLY_01
Western blot analysis of human MCF-7 cell lysate with anti-Keratin K18 antibody. Western blot analysis was performed on 10 μg or 5 μg of MCF-7 lysate. Cells were lysed in PBS by homogenization. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-Keratin K18 mouse monoclonal, Ks18.04 (Cat. No. 690028) was diluted in blocking buffer (antibody concentration 0.25 μg/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goat polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
1NLY_04
IHC of bovine kidney (courtesy of J.Heß, University Hospital Heidelberg)


Geen medisch hulpmiddel / Geen ivD-product
anti-Keratin K18 mouse monoclonal, Ks18.04
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Prijs Hoeveelheid
1NLY.1 0,2 ml plastic

103,10 €

1NLY.2 1 ml plastic

267,70 €

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Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

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