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anti-Keratin K20 mouse monoclonal, IT-Ks20.8, 0.2 ml
Bitte beachten: Abweichende Konditionen / Please note: Different conditions
Boiling point (bp) ~100 °C
Storage temp. +4 °C
Transport temp. ambient temp.
107,40 €/VE
Excl. btw | 0,2 ml Per VE
Bestelnr. 1NNP.1
Productdetails
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-Keratin K20 mouse monoclonal, IT-Ks20.8 50 μg/ml, monoclonal, affinity purified
IT-Ks20.8 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.
Polypeptide recognized: protein IT (keratin K20; Mr 46,000; formerly also designated cytokeratin 20).
Tested cultured cell lines: RT-4
Host: mouse
Antibody type: monoclonal
Isotype: IgG2a
Clone: IT-Ks20.8
Immunogen: electrophoretically purified keratin K20 from human intestinal mucosa
UniprotID: P35900 (human), Q9D312 (mouse)
Synonym: keratin, type I cytoskeletal 20, cytokeratin-20, CK-20, keratin-20, K20, protein IT, KRT20
Conjugate: unconjugated
Purification: affinity chromatography
Intended use: research use only
Application: IHC, WB
Reactivity: human, mouse
Immunohistochemistry (IHC) - frozen: 1:10-1:100 (treatment with 0.02 % Triton X-100 recommended)
Immunohistochemistry (IHC) - paraffin: 1:10-1:100 (protease treatment and/or microwave treatment recommended)
Western Blot (WB): 1:1,000-1:5,000
- Tussentotaal: 0.00
| Bestelnr. | VE | Verp. | Prijs | Hoeveelheid | |
|---|---|---|---|---|---|
| 1NNP.1 | 0,2 ml | plastic |
107,40 € |
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| 1NNP.2 | 1 ml | plastic |
267,70 € |
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- Tussentotaal: 0.00
Downloads / MSDS
Analysecertificaten
Type analysis
| Clonality | Monoclonal |
| Concentration | 50 µg/ml |
| Raised in | Hybridoma cell culture supernatant |
| Immunglobulin (Ig) class | IgG2a |
| Clone | IT-Ks20.8 |
| Conjugate | Unconjugated |
| Purification | Affinity chromatography |
| Purity (SDS-PAGE) | ≥95 % |
| PBS buffer, pH 7.4 with 0.09 % sodium azide and 0.5 % BSA., Short term at 2-8 °C (+4 °C); long term storage in aliquots at -20 °C; avoid freeze/thaw cycles. | |