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ROTI®Cell Glutamine solution
Sterile products
Storage temp. +4 °C
Transport temp. ambient temp.
Solution in cell culture grade water.
As in the case of normal L-glutamine, the recommended final concentration in media is between 292 mg/l (2 mM) and 585 mg/l (4 mM).
Stable glutamine is present as the dipeptide L-alanyl-L-glutamine. Due to its reinforced structure, this form of glutamine is not temperature sensitive, unlike normal L-glutamine. Cells are able to break down this dipeptide bond and release L-glutamine as needed. Thus, the low ammonia concentration improves cell viability and growth. In addition, the concentration of L-glutamine is kept constant.
ROTI®Cell Glutamine Solution can be stored at room temperature for a long period of time.
30,00 €/VE
Excl. btw | 100 ml Per VE
Bestelnr. 9183.1
Productdetails
ROTI®Cell Glutamine solution CELLPURE® 200 mM, sterile, stable
L-glutamine is an essential amino acid that is an important source of energy in cell culture media and is indispensable for cell growth. Since normal L-glutamine is temperature sensitive and spontaneously decomposes to toxic ammonia in the course of cell cultivation, the stable ROTI®Cell Glutamine Solution is an optimal alternative.
Stable glutamine is present as the dipeptide L-alanyl-L-glutamine. Due to its reinforced structure, this form of glutamine is not temperature sensitive, unlike normal L-glutamine. Cells are able to break down this dipeptide bond and release L-glutamine as needed. Thus, the low ammonia concentration improves cell viability and growth. In addition, the concentration of L-glutamine is kept constant.
ROTI®Cell Glutamine Solution can be stored at room temperature for a long period of time.
- Tussentotaal: 0.00
| Bestelnr. | VE | Verp. | Prijs | Hoeveelheid | |
|---|---|---|---|---|---|
| 9183.1 | 100 ml | plastic |
30,00 € |
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- Tussentotaal: 0.00
Downloads / MSDS
Algemene informatie
Our amino acids are of the highest purity and suitable for a broad range of applications in biochemistry. Besides the natural occurring L-amino acids, we offer a selection of unnatural D-amino acids and DL-amino acids.
In addition to the various nutrients in the medium, the pH value also plays a very important role in cell culture. The physiological pH for almost all mammalian cells is 7.4. Deviating from this, it has been observed that certain transformed cell lines prefer a pH between 7.0 and 7.4 and certain fibroblasts prefer a pH between 7.4 and 7.7. In any case, pH fluctuations should be avoided and the pH should be maintained as well as possible. That is why a buffer system in media is necessary. The most important buffer system in the body is the carbonic acid-carbonate buffer system. Accordingly, almost all cell culture media contain bicarbonate (NaHCO3). Bicarbonate alone leads to a strong pH increase of the medium, so that NaHCO3-buffered media must always be used with CO2 incubators. Depending on the NaHCO3 concentration in the medium, different CO2 environments are necessary to ensure a pH of about 7.4. For this reason, you should not work outside of the CO2 incubator for too long. If this is necessary, a HEPES buffer should be used in addition to the NaHCO3, which contributes as a second buffer system to stabilize the pH value.
Analysecertificaten
Type analysis
| Glutamin, stable | 200 mM |
| pH value | 5,5-7,5 |
| Osmolality | tested |
| Endotoxins | <10 EU/ml |
| Sterility | complies |