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ROTI®Quant universal, 500 assays (cuvettes)

for biochemistry
Mogelijke gevolgen: vernietigen metalen en zijn bijtend voor lichaamsweefsel; ernstig oogletsel is mogelijk. Veiligheid: contact vermijden; veiligheidsbril en handschoenen dragen. Bij contact ogen en huid met water spoelen.
Mogelijke gevolgen: zijn voor waterorganismen schadelijk, giftig of zeer giftig, acuut of met langdurige werking. Veiligheid: alleen met gevaarlijk afval afvoeren, in geen geval in het milieu laten terechtkomen.
Danger
H315-H318-H411
i causes skin irritation, causes serious eye damage, toxic to aquatic life with long lasting effects
P273 P280 P305+P351+P338 P310
i avoid release to the environment, wear protective gloves/eye protection, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, immediately call a doctor
VE
Verp.
Verpakking
Biuret solutions
Density (D) 1,37 g/cm³
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
ADR 9 III
WGK 2
UN-Nr. 3082

For protein quantitation (PCA enhanced Biuret reaction). High tolerance against detergents, salts and alcohols.
Kit contents are adequate for 500/200 preparations in photometer cuvettes or 5000/2000 preparations in microtiter plates.

Colorimetric test for detecting and quantifying soluble proteins. Universally applicable.
Productdetails
Notes on the product
201,60 € /VE

151,15 €/VE  Campaign price!

Excl. btw | 1 kit Per VE

Bestelnr. 0120.1

Op voorraad
Vrij van verzendingskosten vanaf 125 €
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Productdetails


Colorimetric test for detecting and quantifying soluble proteins. Universally applicable.

  • Equals the well-known BCA assay
  • Very easy to handle
  • Highly sensitive measurement up to 0.5 µg protein
  • Linear measuring range from 0.5 to 200 µg protein
  • Can be used in 1-ml-scales and in microtiter plates (can be automated)
  • Measurement with standard filters (492 nm wave length) or at 503 nm wave length
  • Accelerated reaction and increased sensitivity in mild heat

The special reagent formula effects a high tolerance against interfering factors such as detergents, alcohol and buffer salts, thus making the kit a universal tool for measuring proteins in almost every solution.


The basic mechanism, a PCA enhanced Biuret reaction, is identical with the one used for the well known BCA protein assay. Its result, however, is a bright blue colour which may be measured with standard filters of approx. 500 nm wave length.



ROTI®Quant universal for biochemistry
Mechanisme

ROTI®Quant universal is based on a biuret reaction in combination with a highly specific colorimetric enhancer reaction. The Cu++ ions of reagent 2 are reduced to Cu+ ions in the alkaline reaction media of the test. The colour development of the copper-specific enhancer reaction is directly proportional to the concentration of the protein which is present. The resulting purple-coloured copper complex exhibits a strong light absorption with a maximum of 503 nm, although the wide maximum of the absorption curve also permits highly sensitive and no-loss measurements at 492 nm. The reaction does not form a true end point measurement: the colour develops slowly at room temperature, this, however, can be accelerated in heat. High sensitivity, therefore, can be achieved even after a short time.

0120_Diagramm_AB
Fig. A: ROTI®Quant universal, standard curve with BSA (bovine serum

albumin). Blank-value corrected. Measurement of 100 µl each protein

solution.

Fig. B: Independence of ROTI®Quant-universal-assay of detergents and other additives, examples. Blank-value corrected. Measurement of 100 µl each protein solution.
(With kind permission of Dr. Angelika Böttger, Zoological Institute, Ludwig-Maximilians-University Munich)

De kit bevat

500/200 ml ROTI®Quant universal reagent 1 (Art. No. 0118) and 40/16 ml ROTI®Quant universal reagent 2 (Art. No. 0119).
Contents of this Kit may not be bought separately.

0120_Diagramm_D
Fig. D: Independence of ROTI®Quant-universal assay of SDS. Microassay in microtiterplates (60 min, 37 °C).


Technische informatie
Bewijs Biuret reaction/PCA (equals BCA) 
ROTI®Quant universal
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Verpakking Prijs Hoeveelheid
0120.1 1 kit plastic 500 assays (cuvettes) 151,15 €
0120.2 1 kit plastic 200 assays (cuvettes) 71,90 €
Op voorraad
Binnenkort beschikbaar
Niet beschikbaar
Leveringsdatum onbekend op dit moment
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

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Notes on the product

Tested for protein gel staining.

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