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Blotting-Sandwich ROTI® ProBlot PVDF 0.2
Van  Carl ROTH
Icon_ready-to-use Ready-to-use products and reagents
Materiaal: polyvinylidenfluoride (PVDF).
Verwante producten
Afmeting


Blotting Sandwich – Simple & Efficient

Our blotting sandwiches offer a time-saving and reliable solution for the efficient transfer of proteins and nucleic acids. They consist of perfectly matched, pre-cut components, including our high-quality ROTI®ProBind membranes, which ensure excellent binding capacity and minimal background interference.
The easy handling reduces sources of error, optimizes reproducibility, and simplifies the workflow. This allows you to achieve consistent and precise results—without any additional preparation effort. Ideal for any laboratory!
Productdetails

279,50 €/VE 

Excl. btw | 20 stuks Per VE

Bestelnr. 3A86.1

Op voorraad
Snelle en betrouwbare levering!
Vrij van verzendingskosten vanaf 250 €
Vanaf 6 VE 265,52 €/VE
Vanaf 24 VE 251,55 €/VE

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Productdetails

Materiaal: polyvinylidenfluoride (PVDF).

Blotting Sandwich – Simple & Efficient

Our blotting sandwiches offer a time-saving and reliable solution for the efficient transfer of proteins and nucleic acids. They consist of perfectly matched, pre-cut components, including our high-quality ROTI®ProBind membranes, which ensure excellent binding capacity and minimal background interference.
The easy handling reduces sources of error, optimizes reproducibility, and simplifies the workflow. This allows you to achieve consistent and precise results—without any additional preparation effort. Ideal for any laboratory!



Blotting-Sandwich ROTI® ProBlot PVDF 0.2 

Toepassingsgebieden:
  • Western Blotting
  • Southern Blotting
  • Northern Blotting
  • Amino Acid and Protein analysis


Geen medisch hulpmiddel / Geen ivD-product
Technische informatie
Poriëngr. 0,2 µm
Binding hydrophobic 
Bindingscapaciteit ≥260 µg/cm²
Dikte 180±20 µm
Immobilisering not necessary 
Materiaal polyvinylidenfluoride 
Blotting-Sandwich ROTI® ProBlot PVDF 0.2
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. Afmeting VE Prijs Hoeveelheid
3A86.1 70 × 84 mm 20 stuks

279,50 €

3A88.1 20 stuks

387,00 €

Op voorraad
Beschikbaar (let op levertijd)
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS

Geen documenten beschikbaar

Algemene informatie

Technical Parameters of ROTH-Transfer Membranes

Membrane Material Characteristics Thickness (μm) Binding capacity (μg/cm2) Pore size (μm) Immobilisation
Roti® Fluoro PVDF Polyvinyliden fluoride hydrophobic 125 ±25 300 0,2 not necessary
Transfer membrane ROTI®PVDF 0.2 Polyvinyliden fluoride hydrophobic 190 ±50 ≥125 0,2 not necessary
Transfer membrane ROTI®PVDF 0.45 Polyvinyliden fluoride hydrophobic 190 ±50 ≥125 0,45 not necessary
Immobilon P Polyvinyliden fluoride hydrophobic 120 ±25 150-300 0,45 not necessary
Roti® Nylon 0.2 Nylon 6,6 neutral/amphoteric 150 ±20 500 0,2/0,45 UV, baking
Roti® Nylon plus Nylon 6,6 hydrophilic, positively charged 160 ±20 500 0,45 not necessary, UV and baking possible
Transfer membrane ROTI®NC 0.2 Nitrocellulose hydrophobic 150 ±50 100 0,2 UV, baking (max. 80 °C)
Transfer membrane ROTI®NC 0.45 Nitrocellulose hydrophobic 150 ±50 100 0,45 UV, baking (max. 80 °C)
Amersham Protran® Nitrocellulose electrostatic, hydrophobic unknown 11-72 0,2/0,45 UV, baking (max. 80 °C)
Amersham Protran® supported Nitrocellulose electrostatic, hydrophobic unknown 86-110 0,2/0,45 UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores Size (kD)
genomic DNA (human) >11000000
E. coli approx. 1500000
0.45 μm approx. 5000
0.2 μm approx. 2000
DNA 3 kb approx. 1000
RNA 1 kb approx. 300
Antibodies (IgG) approx. 150
Proteins in the lab approx. 10–300
BSA (Albumin) 69

Application of ROTH-Transfer Membranes

Biomolecules Nylon plus Nylon 0.2 PVDF 0.2 PVDF Fluoro PVDF NC NC 0.45
DNA +++ + - - - + +
RNA + +++ - - - + +
Proteins - + +++ +++ +++ + +
Detection System
Colour +++ +++ +++ +++ +++ +++ +++
Radioactivity +++ +++ +++ +++ +++ +++ +++
Chemoluminescence +++ +++ +++ +++ - + +
Fluorescence +++ +++ - - +++ - -
+++: recommended +: suitable -: not