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Transfer membrane ROTI®PVDF 0.2, 300 × 30 cm
Van  Carl ROTH
Materiaal: polyvinylidenfluoride (PVDF).
Verwante producten
Afmeting


PVDF membrane for protein analyses.

ROTI®PVDF is a hydrophobic, microporous polyvinylidene membrane, free from adjuvants and detergents. With this membrane, efficient transfers of proteins can be carried out over a wide molecular weight range.
Productdetails

475,15 €/VE 

Excl. btw | 1 rol(len) Per VE

Bestelnr. 1YYC.1

Op voorraad
Vrij van verzendingskosten vanaf 250 €
Snelle en betrouwbare levering!
Vanaf 6 VE 451,39 €/VE
Vanaf 24 VE 427,64 €/VE

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Productdetails

Materiaal: polyvinylidenfluoride (PVDF).

PVDF membrane for protein analyses.

ROTI®PVDF is a hydrophobic, microporous polyvinylidene membrane, free from adjuvants and detergents. With this membrane, efficient transfers of proteins can be carried out over a wide molecular weight range.


Gebruiksinstructies

The membrane shows superior protein binding parameters and stands out for its particularly low background. It may also be used very well after repeated stripping and harsh washing processes.
This mechanically rigid transfer membrane can be easily cut to the desired gel size and it remains flexible and tear-proof even when dry.
Briefly immerse the membrane membrane in 100 % methanol prior to transfer until no more white areas are visible. This transfer membrane is then compatible with every blotting system.
Due to production processes, the membrane shows two different sides - one smooth and one raw. For optimally distinct bands, please use the smooth side for protein binding.



Transfer membrane ROTI®PVDF 0.2 
Geschikt voor

Optimal for proteins≤70 kDa.
Detection by Chemoluminescence, radioactivity, colour.

Toepassingsgebieden:
  • Western blotting
  • Immuno-blotting
  • Solid phase assays
  • Amino acid or protein analysis


Geen medisch hulpmiddel / Geen ivD-product
Technische informatie
Afmeting 300 × 30 cm 
Uitvoering Roll 
Poriëngr. 0,2 µm
Binding hydrophobic 
Bindingscapaciteit 125 µg/cm²
Dikte 190±50 µm
Immobilisering not necessary 
Transfer membrane ROTI®PVDF 0.2
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. Afmeting VE Prijs Hoeveelheid
1YYC.1 300 × 30 cm 1 rol(len)

475,15 €

23H9.1 9 × 10 cm 10 blad

152,20 €

23HC.1 20 × 20 cm 5 blad

163,40 €

23HH.1 7 × 8,4 cm 10 blad

118,55 €

Op voorraad
Beschikbaar
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS

Geen documenten beschikbaar

Algemene informatie

Technical Parameters of ROTH-Transfer Membranes

Membrane Material Characteristics Thickness (μm) Binding capacity (μg/cm2) Pore size (μm) Immobilisation
Roti® Fluoro PVDF Polyvinyliden fluoride hydrophobic 125 ±25 300 0,2 not necessary
Transfer membrane ROTI®PVDF 0.2 Polyvinyliden fluoride hydrophobic 190 ±50 ≥125 0,2 not necessary
Transfer membrane ROTI®PVDF 0.45 Polyvinyliden fluoride hydrophobic 190 ±50 ≥125 0,45 not necessary
Immobilon P Polyvinyliden fluoride hydrophobic 120 ±25 150-300 0,45 not necessary
Roti® Nylon 0.2 Nylon 6,6 neutral/amphoteric 150 ±20 500 0,2/0,45 UV, baking
Roti® Nylon plus Nylon 6,6 hydrophilic, positively charged 160 ±20 500 0,45 not necessary, UV and baking possible
Transfer membrane ROTI®NC 0.2 Nitrocellulose hydrophobic 150 ±50 100 0,2 UV, baking (max. 80 °C)
Transfer membrane ROTI®NC 0.45 Nitrocellulose hydrophobic 150 ±50 100 0,45 UV, baking (max. 80 °C)
Amersham Protran® Nitrocellulose electrostatic, hydrophobic unknown 11-72 0,2/0,45 UV, baking (max. 80 °C)
Amersham Protran® supported Nitrocellulose electrostatic, hydrophobic unknown 86-110 0,2/0,45 UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores Size (kD)
genomic DNA (human) >11000000
E. coli approx. 1500000
0.45 μm approx. 5000
0.2 μm approx. 2000
DNA 3 kb approx. 1000
RNA 1 kb approx. 300
Antibodies (IgG) approx. 150
Proteins in the lab approx. 10–300
BSA (Albumin) 69

Application of ROTH-Transfer Membranes

Biomolecules Nylon plus Nylon 0.2 PVDF 0.2 PVDF Fluoro PVDF NC NC 0.45
DNA +++ + - - - + +
RNA + +++ - - - + +
Proteins - + +++ +++ +++ + +
Detection System
Colour +++ +++ +++ +++ +++ +++ +++
Radioactivity +++ +++ +++ +++ +++ +++ +++
Chemoluminescence +++ +++ +++ +++ - + +
Fluorescence +++ +++ - - +++ - -
+++: recommended +: suitable -: not