ROTI®Free Stripping Buffer 2.0, 250 ml
Boiling point (bp) 100 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
Suitable for nitrocellulose and PVDF-membranes.
Excl. btw | 250 ml Per VE
Now recurring orders conveniently delivered as a subscription!
With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!How it works:
Put all products for your subscription in the desired quantity in the basket.
In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.
Set Starty point and interval for your subscription and submit order!
By the way: Through your account you can customize or delete your subscriptions anytime.
Do you have a question about this product?
ROTI®Free Stripping Buffer 2.0 ready-to-use
Stripping buffer for efficient removal of primary and secondary antibodies, as well as chemoluminescence staining, from Western blot membranes. Due to the low pH of the buffer, the antibody conformation is altered, therefore gently removing all molecules bound to the epitopes (Legocki et. al. (1981) Analyt. Biochem. 111:385-92). Robust formulation without β-mercaptoethanol or DTT for use at room temperature. Suitable for nitrocellulose and PVDF membranes.
- Simple application w/o heating
Following rinsing steps in PBST- or TBST buffer, the membrane is incubated in ROTI®Free Stripping-Puffer 2.0 for 15-60 mins. at room temperature. After washing it again, the membrane may be blocked and probed anew. The particular time needed for complete stripping depends on the respective antibody/epitope pairing.
- Tussentotaal: 0.00
|3319.1||250 ml||glass||42,50 €||
|3319.2||1 l||glass||110,20 €||
Levertijd op aanvraag