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ROTI®Free Stripping Buffer 2.0, 250 ml

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warning
H319
i causes serious eye irritation
P280 P305+P351+P338 P337+P313
i wear protective gloves/protective clothing/eye protection/face protection, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, if eye irritation persists: Get medical advice/attention
Verp.
Pack.
Stripping Buffer
Density (D) 1,003 g/cm³
Boiling point (bp) 100 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
WGK 1

Premixed buffer for gentle stripping of Western-Blot membranes after Chemoluminescence detection.
Suitable for nitrocellulose and PVDF-membranes.

Stripping buffer for efficient removal of primary and secondary antibodies, as well as chemoluminesc ...
Productdetails

42,50 €/VE 

Excl. btw | 250 ml Per VE

Bestelnr. 3319.1

In stock
Vrij van verzendingskosten vanaf 125 €
24-Uur verzending
Vanaf 6 VE 40,38 €/VE
Vanaf 24 VE 38,25 €/VE
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Productdetails



ROTI®Free Stripping Buffer 2.0 ready-to-use

Stripping buffer for efficient removal of primary and secondary antibodies, as well as chemoluminescence staining, from Western blot membranes. Due to the low pH of the buffer, the antibody conformation is altered, therefore gently removing all molecules bound to the epitopes (Legocki et. al. (1981) Analyt. Biochem. 111:385-92). Robust formulation without β-mercaptoethanol or DTT for use at room temperature. Suitable for nitrocellulose and PVDF membranes.

  • Effective
  • Gentle
  • Odour-free
  • Simple application w/o heating
  • Cost-efficient

Gebruiksinstructies

Following rinsing steps in PBST- or TBST buffer, the membrane is incubated in ROTI®Free Stripping-Puffer 2.0 for 15-60 mins. at room temperature. After washing it again, the membrane may be blocked and probed anew. The particular time needed for complete stripping depends on the respective antibody/epitope pairing.



ROTI®Free Stripping Buffer 2.0
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Bestelnr. Verp. Pack. Prijs Hoeveelheid
3319.1 250 ml glass 42,50 €
3319.2 1 l glass 110,20 €
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