Naar de inhoud springen Naar het navigatiemenu springen

ROTI®Free Stripping Buffer, 1 l

ready-to-use
Icon_ready-to-use Ready-to-use products and reagents
Mogelijke gevolgen: leiden tot schade aan de gezondheid, irriteren organen, huid of luchtwegen. Leiden in grotere hoeveelheden tot de dood. Veiligheid: zoals hierboven; bij huidirritaties of oogcontact met water of geschikt middel spoelen.
Warning
H317-H319-H412
i may cause an allergic skin reaction, causes serious eye irritation, harmful to aquatic life with long lasting effects
P280 P302+P352 P305+P351+P338
i wear protective gloves/eye protection, IF ON SKIN: Wash with plenty of water, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
VE
Verp.
Stripping Buffer
Density (D) 1 g/cm³
Boiling point (bp) 100 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
WGK 2

Premixed buffer for stripping of Western-Blot membranes.
Suitable for nitrocellulose and PVDF-membranes.

The buffer offers a simple tool for removing antibodies and chemoluminescence staining from Western blot membranes, allowing the valuable membranes to be analysed several times. Excellent when several proteins are to be detected in the same extract, or e.g. when in addition to a specific analysis, proteins in the respective lanes have to be semi-quantified (Kaufmann et. al. (1987) Anal. Biochem. 161:89-95). Suitable for nitrocellulose and PVDF-membranes.
Productdetails

65,05 €/VE 

Excl. btw | 1 l Per VE

Bestelnr. 0083.1

Op voorraad
Vrij van verzendingskosten vanaf 125 €
Snelle en betrouwbare levering!
Vanaf 6 VE 61,80 €/VE
Vanaf 24 VE 58,54 €/VE
Carl Roth abonnementservice

Now recurring orders conveniently delivered as a subscription!

With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!

How it works:
1

Put all products for your subscription in the desired quantity in the basket.

2

In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.

3

Set Starty point and interval for your subscription and submit order!

By the way: Through your account you can customize or delete your subscriptions anytime.

Productdetails



ROTI®Free Stripping Buffer ready-to-use

The buffer offers a simple tool for removing antibodies and chemoluminescence staining from Western blot membranes, allowing the valuable membranes to be analysed several times. Excellent when several proteins are to be detected in the same extract, or e.g. when in addition to a specific analysis, proteins in the respective lanes have to be semi-quantified (Kaufmann et. al. (1987) Anal. Biochem. 161:89-95). Suitable for nitrocellulose and PVDF-membranes.

  • Efficient
  • Well-tried formulation
  • With β-mercaptoethanol
  • For stripping under heating
  • Low-priced

Gebruiksinstructies

Wash the membrane in TBST-buffer (Art. No. 1061.1), incubate in ROTI®Free Stripping-buffer in closed hood for 30 min at 56 °C (e. g. in shaking water bath). To strip AP-conjugates, we recommend incubation at 70 °C for 30-60 min. After washing the membrane twice in TBST for 20 min each time, it should first be blocked, before the first antibody is added. The stripping process can be checked by brief chemiluminescence staining after washing in TBST.
A slight reduction of the membrane-bound proteins and increase of the background may appear after repeated stripping. Store membranes after stripping wetpacked at +2 to +8 °C.



ROTI®Free Stripping Buffer
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Prijs Hoeveelheid
0083.1 1 l glass 65,05 €
0083.2 250 ml glass 21,00 €
0083.3 2,5 l glass 114,50 €
Op voorraad
Binnenkort beschikbaar
Niet beschikbaar
Leveringsdatum onbekend op dit moment
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / VIB


Analysecertificaten

Hier kunt u uw analysecertificaat voor het geselecteerde product zoeken en downloaden. Geef een chargenummer op.
De volgende analysecertificaten werden gevonden:

Notes on the product

Remark: Contains SDS. Can be redissolved by heating (max. 60 °C).

0 Beoordelingen

0.0 van 5 sterren