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ROTI®Free Stripping Buffer, 1 l

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warning
H317-H319-H412
i may cause an allergic skin reaction, causes serious eye irritation, harmful to aquatic life with long lasting effects
P280.7 P302+P352.0 P305+P351+P338
i wear protective gloves/eye protection, IF ON SKIN: Wash with plenty of water, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Verp.
Pack.
Stripping Buffer
Density (D) 1 g/cm³
Boiling point (bp) 100 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
WGK 2

Premixed buffer for stripping of Western-Blot membranes.
Suitable for nitrocellulose and PVDF-membranes.

The buffer offers a simple tool for removing antibodies and chemoluminescence staining from Western  ...
Productdetails
Type analysis

60,10 €/VE 

Excl. btw | 1 l Per VE

Bestelnr. 0083.1

In stock
Vrij van verzendingskosten vanaf 125 €
24-Uur verzending
Vanaf 6 VE 57,10 €/VE
Vanaf 24 VE 54,09 €/VE
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Productdetails



ROTI®Free Stripping Buffer ready-to-use

The buffer offers a simple tool for removing antibodies and chemoluminescence staining from Western blot membranes, allowing the valuable membranes to be analysed several times. Excellent when several proteins are to be detected in the same extract, or e.g. when in addition to a specific analysis, proteins in the respective lanes have to be semi-quantified (Kaufmann et. al. (1987) Anal. Biochem. 161:89-95). Suitable for nitrocellulose and PVDF-membranes.

  • Efficient
  • Well-tried formulation
  • With β-mercaptoethanol
  • For stripping under heating
  • Low-priced

Gebruiksinstructies

Wash the membrane in TBST-buffer (Art. No. 1061.1), incubate in ROTI®Free Stripping-buffer in closed hood for 30 min at 56 °C (e.g. in shaking water bath). To strip AP-conjugates, we recommend incubation at 70 °C for 30-60 min. After washing the membrane twice in TBST for 20 min each time, it should first be blocked, before the first antibody is added. The stripping process can be checked by brief chemiluminescence staining after washing in TBST.
A slight reduction of the membrane-bound proteins and increase of the background may appear after repeated stripping. Store membranes after stripping wetpacked at +2 to +8 °C.



ROTI®Free Stripping Buffer
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. Verp. Pack. Prijs Hoeveelheid
0083.1 1 l glass 60,10 €
0083.2 250 ml glass 19,90 €
0083.3 2,5 l glass 107,40 €
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Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

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Analysecertificaten

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Type analysis

Remark: Contains SDS. Can be redissolved by heating (max. 45 °C).

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