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Bromocresol green, 25 g

ACS
VE
Verp.
3,3',5,5'-Tetrabrom-m-cresolsulfonphthalein, BCG
Empirical formula C21H14Br4O5S
Molar mass (M) 698,04 g/mol
Melting point (mp) 218 °C
WGK 1
CAS No. [76-60-8]
EG-Nr. 200-972-8

Indicator pH 3.8-5.4
Dissolve 0.1 g in 100 ml ethanol (20 %) or 0.04 g in 0.58 ml NaOH 0.1 mol/l and fill up with water to 100 ml

102,70 €/VE 

Excl. btw | 25 g Per VE

Bestelnr. T115.4

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Vanaf 6 VE 97,56 €/VE
Vanaf 24 VE 92,43 €/VE
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Productdetails



Bromocresol green ACS

Technische informatie
Kleuromslag yellow - blue 
Omslagbereik pH 3,8 - 5,4 
Bromocresol green
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Prijs Hoeveelheid
T115.1 1 g glass

19,25 €

T115.2 5 g glass

31,75 €

T115.3 10 g glass

54,30 €

T115.4 25 g glass

102,70 €

Op voorraad
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS


Algemene informatie

Determination of Nitrogen according to Kjeldahl

The most common technique for measuring the amount of nitrogen in organic matter is the Kjeldahl method, which is used in a wide range of sectors such as environmental analysis, food analysis, water analysis and agricultural analysis, as well as in the pharmaceutical and chemical industries. In this traditional method, a precisely weighed sample is broken down using concentrated sulphuric acid, a process which digests its organic contents and reacts nitrogen to form ammonium sulphate.(CHNO)(s) → CO2 (g) + SO2 (g) + H2O (g) + NH4SO4 (solv, H2SO4)A catalyst or catalyst mixture consisting of copper, selenium, mercury and/or titanium is added to speed up the reaction. Sodium or potassium sulphate is used to give the sulphuric acid a higher boiling point. If the nitrogen is contained in a nitro, nitroso or azo compound, however, the mixture must be reduced with zinc before digestion takes place.
The nitrogen is now present in the sulphuric acid as ammonium sulphate. Adding a strong base (such as NaOH) neutralises the sulphuric acid and liberates ammonia from the solution.NH4SO4 (solv) + 2 NaOH (aq) → Na2SO4 (aq) + 2 NH3 (g) + 2 H2O (l) The ammonia is led into an acid (such as boric acid) by means of steam distillation.B(OH)3 (aq) + 2 H2O (l) + NH3 (g) → B(OH)4- (aq) + NH4+ (aq) The resulting strong base (borate ion) is back-titrated with a strong acid (hydrochloric acid or sulphuric acid). The excess weak boric acid is not captured in the process. A Tashiro's indicator that changes colour in the acid is used for titration. The amount of acid that has been used up can then be converted into the amount of nitrogen in the sample. NH4+ (aq) + B(OH)4- (aq) + HCl (l) → NH4Cl (aq) + B(OH)3 (aq) + H2O (l)To calculate the protein content of the sample, the varying nitrogen content of the amino acids must be checked and the relevant conversion factors applied. The nitrogen contained in food derives mainly from proteins, but different samples may also contain other sources of nitrogen.


All SOLVAGREEN® products as well as further information about the product can also be found in our current flyer Green Chemistry - Alternative Solvents and Products.


U vindt nog meer aantrekkelijke aanbiedingen om uw chromatografielaboratorium te vervolledigen op onze Chromatografie pagina!


Thin Layer Chromatography (TLC)

Although the principle of thin-layer chromatography is more than a century old, it did not make its breakthrough as an analytical method until about 50 years ago.
Thanks to the development of new sorbents and supports, as well as increasing instrumentation and automation, TLC has become a versatile separation method. It is used both in qualitative analysis and in quantitative analysis.
Applications range from simple manual separation processes in classic TLC to automated processes in HPTLC (high performance thin layer chromatography).

Advantages of thin layer chromatography:

  • Higher sample throughput in less time
  • Suitable for screening tests
  • Pilot process for HPLC
  • The ready-to-use TLC layer functions as a data storage device for separation results
  • The separated substances can be used later for further analysis (e.g. IR, MS)
  • By switching the mobile and the stationary phases, the separation process can be optimised quickly and cost-efficient


Indicators and Dyes

Carl ROTH offers many indicators and dyes of high purity, which can be used in special applications.


Analysecertificaten

Hier kunt u uw analysecertificaat voor het geselecteerde product zoeken en downloaden. Geef een chargenummer op.
De volgende analysecertificaten werden gevonden:

Guarantee analysis

Appearancelight tan powder
Appearance of solutioncomplies
pH-transition range3.8 (yellow)-5.4 (blue)
Loss on drying (110 °C)≤3 %