product.details.print
ROTI®Cell Pyruvate solution
Storage temp. +4 °C
Transport temp. ambient temp.
Solution in cell culture grade water.
Recommended end concentration 1 mM, in special media up to 5 mM.
21,40 €/VE
Excl. btw | 100 ml Per VE
Bestelnr. 9182.1
Productdetails
ROTI®Cell Pyruvate solution CELLPURE® 100 mM, sterile
Sodium Pyruvate is a key intermediate in the ATP production within the cell and is sometimes added to cell culture media as an additional source of energy. Additional pyruvate may help cells deficient in one of the enzymes involved in glucose metabolism, and may kick-start cells in the citric acid cycle, thus supporting growth of low density cells. Concentration in standard media is 1 mM; special media like Leibovitz’s medium are supplemented with up to 5 mM pyruvate.
- Tussentotaal: 0.00
Bestelnr. | VE | Verp. | Prijs | Hoeveelheid | |
---|---|---|---|---|---|
9182.1 | 100 ml | plastic |
21,40 € |
|
|
Op voorraad
Beschikbaar
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
|
- Tussentotaal: 0.00
Downloads / MSDS
Algemene informatie
In addition to the various nutrients in the medium, the pH value also plays a very important role in cell culture. The physiological pH for almost all mammalian cells is 7.4. Deviating from this, it has been observed that certain transformed cell lines prefer a pH between 7.0 and 7.4 and certain fibroblasts prefer a pH between 7.4 and 7.7. In any case, pH fluctuations should be avoided and the pH should be maintained as well as possible. That is why a buffer system in media is necessary. The most important buffer system in the body is the carbonic acid-carbonate buffer system. Accordingly, almost all cell culture media contain bicarbonate (NaHCO3). Bicarbonate alone leads to a strong pH increase of the medium, so that NaHCO3-buffered media must always be used with CO2 incubators. Depending on the NaHCO3 concentration in the medium, different CO2 environments are necessary to ensure a pH of about 7.4. For this reason, you should not work outside of the CO2 incubator for too long. If this is necessary, a HEPES buffer should be used in addition to the NaHCO3, which contributes as a second buffer system to stabilize the pH value.
Analysecertificaten
Type analysis
Sodium pyruvate | 100 mM |
Osmolality | 170-1240 mOsm/kg |
Endotoxins | ≤10,0 EU/ml |
Sterility | complies |
pH value | tested |