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ROTI®Fect, 0.2 ml

CELLPURE® for transfection
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Verp.
Pack.
Verpakking
Transfection reagents
Storage temp. +4 °C
WGK 3

Reagent for liposoma-mediated transfection of eukaryotic cells.
Approx. 400 assays per ml in 24wells. Dependent on the cell line used.

ROTI®Fect is a liposome formulation of a polycationic lipid in combination wit ...
Productdetails
73,00 € /VE

56,90 €/VE  Campaign price!

Excl. btw | 0,2 ml Per VE

Bestelnr. P001.1

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Vrij van verzendingskosten vanaf 125 €
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Productdetails



ROTI®Fect CELLPURE® for transfection

ROTI®Fect is a liposome formulation of a polycationic lipid in combination with a neutral colipid. Compared to monocationic lipid formulations, ROTI®Fect has a higher efficiency with lower levels being required. Its excellent reproducibility in combination with its high level of efficiency reduced optimisation work to a minimum. Because of its low toxicity, a wide range of cell lines can be transfected efficiently.
An efficient triple-cotransfection using ROTI®Fect is described in Ufartes, Schlüter, Kaulfuss and Seipp (2005) Biospektrum 4: 464-5.

  • Highly efficient reproduction of DNA in mammal cells
  • Excellent reproducibility
  • High and wide operating plateau
  • No inhibition through serum
  • Low cell toxicity
  • Successful with different cell lines
  • Excellent transfection results

Met succes getest op volgende celtypes

siRNA: CEM, COS-7, ES-D3,HeLa, HL60, LNCaP, OVCAR3, PC3, SW620
Primary cells: pAML, pHDF, pMEF, pRHC, pSMC
Cell lines: 143B, 293-T, A-293, A-431, AM-C6SC8, BHK, BV-2, C2C12, C6, CHO, CHO-DHFR, COS-1, COS-7, CRFK, Colo-205, DU-145, ECV-304, EMC, F98 (glioma), GD25-ß1, HBL 100, HCT-116, HCT-15, HEK293, HeLa, HeLa-S3, Hep-3B, Hep-G2, HOSE, HSG, HT-22, HT-29, HUVEC, JURKAT, LLC-PK1, LS174T, LoVo, MCF-7, MDCK, MeWo, MV3, NIH-3T3, NS20Y, OK, P815, PC3, PT-11, Rcho-1, SAOS, SH-SY5Y, SK-MEL-28, SKOV-3, SM10, SW-480, THP-1, tsA201, U87 (glioma), Vero 76, et al.


P001_RotiFect_Ablaufschema
P001_RotiFect_ausBIOspektrum
P001_Diagramm
Figure:
Transfection of a variety of cell lines with ROTI®Fect and other commercially available transfectants.
Transfection was performed in 24wells using 0.25 µg DNA each, with 1.5 µl ROTI®Fect or acc. to instructions given by the manufacturers. Luciferase activity was measured 48 hours post transfection. Data of cells transfected with ROTI®Fect was set to 100 %.


ROTI®Fect
Geselecteerde hoeveelheid:   0
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Bestelnr. Verp. Pack. Verpakking Prijs Hoeveelheid
P001.1 0,2 ml plastic 1 x 0,2 ml 56,90 €
P001.2 0,5 ml plastic 127,30 €
P001.3 1 ml plastic 199,45 €
P001.4 5 ml plastic 5 x 1 ml 862,80 €
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