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ROTI®Aqua-P/C/I, 500 ml

ready-to-use, for RNA extraction
Icon_EVE-EUD Please consider that this product might be liable to some legal sanctions as for example to obtain an end user declaration or the prohibition of some applications. For details please do contact your counterpart in our sales department!
Icon_ready-to-use Ready-to-use products and reagents
Mogelijke gevolgen: vernietigen metalen en zijn bijtend voor lichaamsweefsel; ernstig oogletsel is mogelijk. Veiligheid: contact vermijden; veiligheidsbril en handschoenen dragen. Bij contact ogen en huid met water spoelen.
Mogelijke gevolgen: leiden in kleinere hoeveelheden onmiddellijk tot ernstige schade aan de gezondheid of tot de dood. Veiligheid: niet inademen, aanraken, inslikken. Beschermingsmiddelen dragen. Onmiddellijk antigifcentrum of arts op de hoogte brengen. Stabiele zijpositie.
Mogelijke gevolgen: veroorzaken allergieën, kankerverwekkend (carcinogeen), verandering erfelijk materiaal (mutageen), brengt de voortplanting in gevaar en vruchtbeschadigend (reprotoxisch) of orgaanbeschadigend Veiligheid: voor het werk met dergelijke stoffen moet men zich goed informeren; veiligheidskleding en handschoenen, oog- en mondbescherming of adembescherming dragen.
Mogelijke gevolgen: zijn voor waterorganismen schadelijk, giftig of zeer giftig, acuut of met langdurige werking. Veiligheid: alleen met gevaarlijk afval afvoeren, in geen geval in het milieu laten terechtkomen.
Danger
H301+H331-H312-H314-H341-H351-H361d-H372-H411
i toxic if swallowed or if inhaled, harmful in contact with skin, causes severe skin burns and eye damage, suspected of causing genetic defects, suspected of causing cancer, suspected of damaging the unborn child, causes damage to organs through prolonged or repeated exposure, toxic to aquatic life with long lasting effects
P201 P280 P304+P340 P305+P351+P338 P308+P313
i obtain special instructions before use, wear protective clothing/eye protection/face protection, IF INHALED: Remove person to fresh air and keep comfortable for breathing, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, IF exposed or concerned: Get medical advice/attention
VE
Verp.
Phenol solutions
Density (D) 1,26 g/cm³
Boiling point (bp) >65 °C
Storage temp. +4 °C
ADR 6.1 II
WGK 3
UN-Nr. 2821

Redistilled, in water saturated phenol, chloroform and isoamyl alcohol at a ratio of 25:24:1, pH 4,5-5.
Store in the dark!

ROTI®Aqua-P/C/I is the optimal choice for RNA isolation and other procedures where a low phenol pH value is desired.
The low pH results in interphase enrichment of the DNA, reducing DNA contamination in the RNA eluate.
Productdetails

83,35 €/VE 

Excl. btw | 500 ml Per VE

Bestelnr. X985.2

Binnenkort beschikbaar
Vrij van verzendingskosten vanaf 125 €
Vanaf 6 VE 79,18 €/VE
Vanaf 24 VE 75,02 €/VE
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Productdetails


  • Reduce exposure to toxic chemicals
  • Prepared from phenol of highest purity
  • Packed under argon for maximum stability
  • Successfully tried and tested in many research laboratories


Please note our Technical Information Brochure on the Internet next to the products:

Phenolic DNA Isolation
Background and protocol


Mechanism

Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses.
For distinct phase separation, ROTI®Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl.

Draw solution from lower phase. Do not shake before use!



ROTI®Aqua-P/C/I ready-to-use, for RNA extraction

ROTI®Aqua-P/C/I is the optimal choice for RNA isolation and other procedures where a low phenol pH value is desired.
The low pH results in interphase enrichment of the DNA, reducing DNA contamination in the RNA eluate.


Mechanisme

Addition of 1 volume ROTI®Aqua-P/C/I and thorough mixing. Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. The acid pH-value causes a depletion of the DNA, so that only little contaminating DNA is isolated. Following centrifugation, the RNA containing solution may be retrieved as upper phase.
For distinct phase separation, ROTI®Aqua-P/C/I needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 12 drops of 1 N HCl.



ROTI®Aqua-P/C/I
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Prijs Hoeveelheid
X985.1 250 ml glass 54,30 €
X985.2 500 ml glass 83,35 €
X985.3 100 ml glass 31,10 €
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Binnenkort beschikbaar
Niet beschikbaar
Leveringsdatum onbekend op dit moment
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

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