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ROTI®Sep 1077 human, 100 ml

CELLPURE® sterile, ready-to-use, for density gradient centrifugation
Icon_Steril Sterile products
Icon_ready-to-use Ready-to-use products and reagents
Mogelijke gevolgen: veroorzaken allergieën, kankerverwekkend (carcinogeen), verandering erfelijk materiaal (mutageen), brengt de voortplanting in gevaar en vruchtbeschadigend (reprotoxisch) of orgaanbeschadigend Veiligheid: voor het werk met dergelijke stoffen moet men zich goed informeren; veiligheidskleding en handschoenen, oog- en mondbescherming of adembescherming dragen.
Danger
H317-H334
i may cause an allergic skin reaction, may cause allergy or asthma symptoms or breathing difficulties if inhaled
P302+P352 P333+P313
i IF ON SKIN: Wash with plenty of water, if skin irritation or rash occurs: Get medical advice/attention
VE
Verp.
Lymphocyte separation medium, Polysucrose solution
Molar mass (M) ~400000 g/mol
Density (D) 1,08 g/cm³
Boiling point (bp) 100 °C
Storage temp. +4 °C
WGK 3

Polysucrose based medium for separation of lymphocytes/PBMC from human whole blood. Density: 1,077 g/ml
Long-term storage at approx. 4 °C recommended. Short-term storage at room temperature possible. Storage protected from light.

Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymp ...
Productdetails
Type analysis
45,70 € /VE

35,35 €/VE  Campaign price!

Excl. btw | 100 ml Per VE

Bestelnr. 0642.1

Op voorraad
Vrij van verzendingskosten vanaf 125 €
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Productdetails


Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from human blood by density gradient centrifugation.
Density 1.077 g/ml.
Best alternative to Ficoll®.

  • Optimised for isolation of lymphocytes from non-coagulated human whole blood
  • Produces distinct, compact layers
  • Results in high recovery rates of viable cells
  • Maintains the representative ratio of B- to T lymphocytes
  • May be directly applied to all protocols using Ficoll®

ROTI®Sep 1077 human excels due to the superior separation power, forming very distinct, easy to pipet, lymphocyte layers during centrifugation. The ready to use solution is very easy in handling, resulting in high cell recovery rates, while cell viability and the representative ratio of B- to T lymphocytes are maintained. Osmolality as well as pH value are strictly kept in the physiological range.

Suitable for application with human non-coagulated peripheral blood. In all respective protocols originally using Ficoll® this reagent may be replaced by ROTI®Sep 1077 human without any adjustment.



ROTI®Sep 1077 human CELLPURE® sterile, ready-to-use, for density gradient centrifugation

For research use only. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro diagnostics.

0642_02
The figure depicts two specimens prepared with heparinic blood obtained from venous puncture. Density gradient centrifugation of 6 ml diluted whole blood (dilution in PBS 1:1.5), with 3 ml of ROTI®Sep 1077 human, 10 mins., 1000 g, no break.


ROTI®Sep 1077 human
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Prijs Hoeveelheid
0642.1 100 ml plastic 35,35 €
0642.2 500 ml plastic 95,45 €
Op voorraad
Binnenkort beschikbaar
Niet beschikbaar
Leveringsdatum onbekend op dit moment
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

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Analysecertificaten

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De volgende analysecertificaten werden gevonden:

Type analysis

Appearance of solutionclear, colourless  
Density1,077 ±0,001 g/ml  
pH value7,0 ±0,5  
Osmolality325 ±35 mOsm/kg  
Sterilitycomplies  
Endotoxinstested  
For research use only. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro diagnostics.

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