ClickTech EdU Cell Proliferation Kit 488 Sensitive

The copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) is the most prominent example of a group of reactions named click reactions. According to Sharpless’ definition, these reactions are characterised by high yields, mild reaction conditions, and by their tolerance of a broad range of functional groups.^[1-3] Typically, the reactions require simple or no workup or purification of the product. The most important characteristic of the CuAAC reaction is its unique bioorthogonality as neither azide nor terminal alkyne functional groups are generally present in natural systems.

References:
1 H. C. Kolb, M.G. Finn, K. B. Sharpless, Angew. Chem. Int. Ed. 2001, 40, 2004-2021.
2 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064.
3 V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.

In recent years, click chemistry has become increasingly important and steadily more popular in research and development. The reasons for this are not only the easy handling and the high yields but also the countless application possibilities. Carl ROTH offers you the possibility to purchase the broad portfolio of click reagents, various kits for EdU-based cell proliferation assays and numerous other applications from the well-known brand baseclick. This enables us to offer you a necessary and comprehensive assortment for click chemistry.

The number at the end of the product name specifies the excitation wavelength of the included fluorescent marker. Suitable excitation source are:

The ClickTech EdU Cell Proliferation Kits for EdU mediated detection of cell proliferation provide a superior alternative to time-consuming and less sensitive bromodeoxyuridine (BrdU) assays. Similar to BrdU assays, these assays utilise a uracil derivative (in this case ethynyldeoxyuridine, EdU) which is incorporated into the replicating DNA. EdU is not detected via antibodies; instead fluorescent dyes are coupled directly via click reaction. This fast and elegant approach allows for direct labelling of replicating DNA with the desired fluorescent dye. Thus, the assay is perfectly adaptable to the present facilities and to the assay situation (e.g. for double or triple detection). Furthermore, the click reaction is highly selective and therefore prevents any unspecific labelling. The protocol includes only few steps and decreases the total amount of time.

Kits are available for the following applications:

▶ Kits for cell proliferation: • T cell Proliferation (ClickTech EdU T Cell Proliferation Kit - Flow Cytometry) • Fluorescence microscopy (ClickTech EdU Cell Proliferation Kit - Imaging) • High Throughput Screening (ClickTech EdU Cell Proliferation Kit - HTS) • Flow cytometry (ClickTech EdU Cell Proliferation Kit - Flow Cytometry)▶ Kits for nucleic acid modification: • PCR Modification • DNA FISH Kit • RNA Labeling Kit • ClickTech Oligo Link Kit • ClickTech Library Kit - full-length mRNA Seq

Advantages of the click chemistry based approach:

Advantages of the click chemistry based method:

• Highly reliable
• Easy handling
• Fast detection procedure (only 30 min.)
• Mild conditions, no DNA denaturation required
• Modular system in click chemistry
• Compatible with various dyes
• Compatible with multiplexing

The ClickTech EdU Cell Proliferation Kits for Flow Cytometry by baseclick use click chemistry to detect cell proliferation by means of a large number of fluorescent stain read-outs. In the detection procedure both, the total number of steps and the processing time are significantly reduced. The simple click chemistry detection procedure is complete within 30 minutes.

References:
1 N. Dinh Van et al., Modulation of HCV reinfection after orthotopic liver transplantation by fibroblast growth factor-2 and other non-interferon mediators. Gut 2016, 65, 1015-1023.
2 L. Pisarsky et al., Targeting Metabolic Symbiosis to Overcome Resistance to Anti-angiogenic Therapy. Cell Reports 2016, 15, 1-14.