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4-Nitrophenyl phosphate disodium salt hexahydrate, 2.5 g

≥99 %, for biochemistry
VE
Verp.
pNPP, p-Nitrophenyl phosphate
Empirical formula C6H4NNa2O6P · 6 H2O
Molar mass (M) 371,12 g/mol
Melting point (mp) >300 °C
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1
CAS No. 4264-83-9
EG-Nr. 224-246-5

Substrate for alkaline phosphatase, particularly for ELISA.

Substrate of the alkaline phosphatase for use in ELISAs. During reaction of the substrate, a soluble, yellow product is formed that is detected photometrically at 405 nm wave length.
Productdetails

19,90 €/VE 

Excl. btw | 2,5 g Per VE

Bestelnr. 4165.1

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Vanaf 6 VE 18,90 €/VE
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Productdetails



4-Nitrophenyl phosphate disodium salt hexahydrate ≥99 %, for biochemistry

Substrate of the alkaline phosphatase for use in ELISAs. During reaction of the substrate, a soluble, yellow product is formed that is detected photometrically at 405 nm wave length.


Gebruiksinstructies

Working solution: 1 mg/ml in diethanolamine substrate buffer (10 mM diethanolamine, 0.5 mM MgCl2, pH 9.5).
For endpoint analysis the reaction may be stopped via addition of 2-3 M NaOH.



Technische informatie
Enzyme Alkaline phosphatase 
Bewijs Colour (yellow) 
Signaalproduct soluble (ELISA, activity assay) 
4-Nitrophenyl phosphate disodium salt hexahydrate
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Prijs Hoeveelheid
4165.1 2,5 g glass

19,90 €

4165.7 100 g glass

235,45 €

4165.9 25 g glass

84,40 €

Op voorraad
Beschikbaar
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS


Algemene informatie

Assay protocol for use of BCIP/NBT in immunoblot procedures:

Stock solutions (All solutions are stable for at least 1 year at 4 °C):
0.5 g NBT in 10 ml 70 % dimethylformamide;
0.5 g BCIP (p-toluidine salt) in 10 ml 100 % dimethylformamide.
Incubation buffer for alkaline phosphatase:
100 mM NaCl, 5 mM MgCl2,100 mM Tris (pH 9.5).
Fresh substrate solution: 66 μl NBT stock solution + 10 ml incubation buffer, mix well, add 33 μl BCIP stock solution. Use within 1 hour.
Blot development: Approx. 10 ml substrate solution per 15 x 15 cm2 membrane surface. Develop at room temperature until bands become visible (approx. 30 mins).
Reaction stop: Rinse with PBS/20 mM EDTA.


Detection of peroxidase activity (Immunoassay):

Dissolve 1 mg TMB in 0.1 ml dimethylsulfoxide (Art. No. 4720); add 9.9 ml of a 0.1 M sodium acetate solution (pH 6.0) (Powder: Art. No. 6779), filter and add H2O2 (Art. No. 8070) (final concentration 0.01 %).

Always prepare freshly!

Incubation 10-30 mins at room temperature (approx. 50 μl per microtitre well; finally add 50 μl 1 M H2SO4, Art. No. X873, per well).
Photometric quantitation at 450 nm.

Reference: Bos E.S. et al. (1981) J. Immunoassay 2, (3/4), 187.


Analysecertificaten

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De volgende analysecertificaten werden gevonden:

Type analysis

Appearanceoff-white to light-yellow crystalline powder
Assay (HPLC)≥99.0 %
Water27.0-31.5 %
pH value (5 % in water)8.0-10.5