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4-Nitrophenyl phosphate disodium salt hexahydrate, 2.5 g
Molar mass (M) 371,12 g/mol
Melting point (mp) >300 °C
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1
CAS No. 4264-83-9
EG-Nr. 224-246-5
19,90 €/VE
Excl. btw | 2,5 g Per VE
Bestelnr. 4165.1
Productdetails
4-Nitrophenyl phosphate disodium salt hexahydrate ≥99 %, for biochemistry
Substrate of the alkaline phosphatase for use in ELISAs. During reaction of the substrate, a soluble, yellow product is formed that is detected photometrically at 405 nm wave length.
Working solution: 1 mg/ml in diethanolamine substrate buffer (10 mM diethanolamine, 0.5 mM MgCl2, pH 9.5).
For endpoint analysis the reaction may be stopped via addition of 2-3 M NaOH.
Enzyme | Alkaline phosphatase |
Bewijs | Colour (yellow) |
Signaalproduct | soluble (ELISA, activity assay) |
- Tussentotaal: 0.00
Bestelnr. | VE | Verp. | Prijs | Hoeveelheid | |
---|---|---|---|---|---|
4165.1 | 2,5 g | glass |
19,90 € |
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4165.7 | 100 g | glass |
235,45 € |
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4165.9 | 25 g | glass |
84,40 € |
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|
Op voorraad
Beschikbaar
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
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- Tussentotaal: 0.00
Downloads / MSDS
Algemene informatie
Stock solutions (All solutions are stable for at least 1 year at 4 °C):
0.5 g NBT in 10 ml 70 % dimethylformamide;
0.5 g BCIP (p-toluidine salt) in 10 ml 100 % dimethylformamide.
Incubation buffer for alkaline phosphatase:
100 mM NaCl, 5 mM MgCl2,100 mM Tris (pH 9.5).
Fresh substrate solution: 66 μl NBT stock solution + 10 ml incubation buffer, mix well, add 33 μl BCIP stock solution. Use within 1 hour.
Blot development: Approx. 10 ml substrate solution per 15 x 15 cm2 membrane surface. Develop at room temperature until bands become visible (approx. 30 mins).
Reaction stop: Rinse with PBS/20 mM EDTA.
Dissolve 1 mg TMB in 0.1 ml dimethylsulfoxide (Art. No. 4720); add 9.9 ml of a 0.1 M sodium acetate solution (pH 6.0) (Powder: Art. No. 6779), filter and add H2O2 (Art. No. 8070) (final concentration 0.01 %).
Always prepare freshly!
Incubation 10-30 mins at room temperature (approx. 50 μl per microtitre well; finally add 50 μl 1 M H2SO4, Art. No. X873, per well).
Photometric quantitation at 450 nm.
Reference: Bos E.S. et al. (1981) J. Immunoassay 2, (3/4), 187.
Analysecertificaten
Type analysis
Appearance | off-white to light-yellow crystalline powder |
Assay (HPLC) | ≥99.0 % |
Water | 27.0-31.5 % |
pH value (5 % in water) | 8.0-10.5 |