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3,3'-Diaminobenzidine tetrahydrochloride, 1 g, glass

≥98 %, p.a.
Mogelijke gevolgen: leiden tot schade aan de gezondheid, irriteren organen, huid of luchtwegen. Leiden in grotere hoeveelheden tot de dood. Veiligheid: zoals hierboven; bij huidirritaties of oogcontact met water of geschikt middel spoelen.
Mogelijke gevolgen: veroorzaken allergieën, kankerverwekkend (carcinogeen), verandering erfelijk materiaal (mutageen), brengt de voortplanting in gevaar en vruchtbeschadigend (reprotoxisch) of orgaanbeschadigend Veiligheid: voor het werk met dergelijke stoffen moet men zich goed informeren; veiligheidskleding en handschoenen, oog- en mondbescherming of adembescherming dragen.
i causes skin irritation, causes serious eye irritation, may cause respiratory irritation, suspected of causing genetic defects, suspected of causing cancer
P202 P261 P280 P305+P351+P338 P308+P313
i do not handle until all safety precautions have been read and understood, avoid breathing dust, wear protective gloves/eye protection, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, IF exposed or concerned: Get medical advice/attention
DAB, 3,3',4,4'-Tetra-aminobiphenyl tetrahydrochloride
Empirical formula C12H14N4 · 4 HCl
Molar mass (M) 360,10 g/mol
Melting point (mp) >280 °C
Storage temp. +4 °C
CAS No. [7411-49-6]
EG-Nr. 231-018-9

Substrate of horseradish peroxidase. For detection of endogenous peroxidase and for immunohistochemical stainings.
Application: Stock solution: 1-2 % (10-20 mg/ml) in 50 mM Tris buffer, pH 7.3. Storage of stock solution in aliquots at -20 °C. Working concentration: 0.05-0.1 % (0.5-1 mg/ml) DAB in buffer (PBS, TBS, pH 7.0-7.6) containing 0.01 % hydrogen peroxide. Always use freshly prepared working solution. For oxidation high concentrations of fresh hydrogen peroxide are needed.

Popular substrate of peroxidases. DAB is used for biochemical and histological detection of endogeno ...
Type analysis

24,65 €/VE 

Excl. btw | 1 g Per VE

Bestelnr. CN75.1

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Vanaf 6 VE 23,42 €/VE
Vanaf 24 VE 22,18 €/VE
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3,3'-Diaminobenzidine tetrahydrochloride ≥98 %, p.a.

Popular substrate of peroxidases. DAB is used for biochemical and histological detection of endogenous peroxidases like catalase and cytochome oxidase. Additionally, it is the most common substrate for horseradish peroxidase (HRP) in histochemical and cytological assays. DAB is oxidised by HRP, forming a brown precipitate, which is unsoluble in aqueous and organic solvents. This precipitate can be detected in visible light and does not bleach during long-term storage.

Technische informatie
Toepassing Western, in situ, Histochemistry  
Enzyme Meerrettich Peroxidase  
Bewijs Farbe (braun)  
Signaalproduct Präzipitat (Blot)  
3,3'-Diaminobenzidine tetrahydrochloride
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Prijs Hoeveelheid
CN75.1 1 g glass 24,65 €
CN75.2 5 g plastic 79,45 €
CN75.3 10 g plastic 136,00 €
Op voorraad
Binnenkort beschikbaar
Niet beschikbaar
Leveringsdatum onbekend op dit moment
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00


Algemene informatie

Assay protocol for use of BCIP/NBT in immunoblot procedures:

Stock solutions (All solutions are stable for at least 1 year at 4 °C):
0.5 g NBT in 10 ml 70 % dimethylformamide;
0.5 g BCIP (p-toluidine salt) in 10 ml 100 % dimethylformamide.
Incubation buffer for alkaline phosphatase:
100 mM NaCl, 5 mM MgCl2,100 mM Tris (pH 9.5).
Fresh substrate solution: 66 μl NBT stock solution + 10 ml incubation buffer, mix well, add 33 μl BCIP stock solution. Use within 1 hour.
Blot development: Approx. 10 ml substrate solution per 15 x 15 cm2 membrane surface. Develop at room temperature until bands become visible (approx. 30 mins).
Reaction stop: Rinse with PBS/20 mM EDTA.

Detection of peroxidase activity (Immunoassay):

Dissolve 1 mg TMB in 0.1 ml dimethylsulfoxide (Art. No. 4720); add 9.9 ml of a 0.1 M sodium acetate solution (pH 6.0) (Powder: Art. No. 6779), filter and add H2O2 (Art. No. 8070) (final concentration 0.01 %).

Always prepare freshly!

Incubation 10-30 mins at room temperature (approx. 50 μl per microtitre well; finally add 50 μl 1 M H2SO4, Art. No. X873, per well).
Photometric quantitation at 450 nm.

Reference: Bos, E.S. et al., (1981) J. Immunoassay 2, (3/4), 187.


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De volgende analysecertificaten werden gevonden:

Type analysis

Assay (HPLC)≥98 %  
Suitablility for determination of Seleniumcomplies  
Appearance of solutionReddish brown  

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