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3,3',5,5'-Tetramethylbenzidine, 5 g, plastic

≥98 %, p.a.
Empirical formula C16H20N2
Molar mass (M) 240,35 g/mol
Density (D) 0,45 g/cm³
Melting point (mp) 170 °C
Storage temp. +4 °C
CAS No. [54827-17-7]
EG-Nr. 259-364-6

Substrate for peroxidase.
Working solution: 1 mg TMB in 0.1 ml dimethyl sulfoxide, add 9.9 ml 0.1 M sodium acetate (pH 6). Filter and add H2O2 ad 0.01 %. Always prepare freshly!

Application: approx. 50 µl per 96well, incubate for 10-30 mins. at room temperature. Add 50 µl 1 M H2SO4 and quantitate photometrically at 450 nm (acc. to: Bos et. al. (1981) J. Immunoassay 2:187).

Protect from light.

Used as a highly sensitive substrate for peroxidases, particularly in ELISA procedures. Can replace carcinogenic benzidine.

Dissolves easily in acetone, chloroform and ethanol.
Type analysis

170,95 €/VE 

Excl. btw | 5 g Per VE

Bestelnr. 6350.2

Binnenkort beschikbaar
Vrij van verzendingskosten vanaf 125 €
Vanaf 6 VE 162,40 €/VE
Vanaf 24 VE 153,86 €/VE
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3,3',5,5'-Tetramethylbenzidine ≥98 %, p.a.

Used as a highly sensitive substrate for peroxidases, particularly in ELISA procedures. Can replace carcinogenic benzidine.

Dissolves easily in acetone, chloroform and ethanol.

Technische informatie
Enzyme Horseradish Peroxidase 
Bewijs Colour (blue) 
Signaalproduct soluble (ELISA) 
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Prijs Hoeveelheid
6350.1 1 g glass 56,90 €
6350.2 5 g plastic 170,95 €
6350.3 25 g plastic 568,70 €
Op voorraad
Binnenkort beschikbaar
Niet beschikbaar
Leveringsdatum onbekend op dit moment
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00


Algemene informatie

Assay protocol for use of BCIP/NBT in immunoblot procedures:

Stock solutions (All solutions are stable for at least 1 year at 4 °C):
0.5 g NBT in 10 ml 70 % dimethylformamide;
0.5 g BCIP (p-toluidine salt) in 10 ml 100 % dimethylformamide.
Incubation buffer for alkaline phosphatase:
100 mM NaCl, 5 mM MgCl2,100 mM Tris (pH 9.5).
Fresh substrate solution: 66 μl NBT stock solution + 10 ml incubation buffer, mix well, add 33 μl BCIP stock solution. Use within 1 hour.
Blot development: Approx. 10 ml substrate solution per 15 x 15 cm2 membrane surface. Develop at room temperature until bands become visible (approx. 30 mins).
Reaction stop: Rinse with PBS/20 mM EDTA.

Detection of peroxidase activity (Immunoassay):

Dissolve 1 mg TMB in 0.1 ml dimethylsulfoxide (Art. No. 4720); add 9.9 ml of a 0.1 M sodium acetate solution (pH 6.0) (Powder: Art. No. 6779), filter and add H2O2 (Art. No. 8070) (final concentration 0.01 %).

Always prepare freshly!

Incubation 10-30 mins at room temperature (approx. 50 μl per microtitre well; finally add 50 μl 1 M H2SO4, Art. No. X873, per well).
Photometric quantitation at 450 nm.

Reference: Bos, E.S. et al., (1981) J. Immunoassay 2, (3/4), 187.


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De volgende analysecertificaten werden gevonden:

Type analysis

Appearancebeige cryst. powder
Assay≥98,0 %
Sulphated ash≤0,2 %
Loss on drying (3 h, 60 °C, vacuum)≤1,0 %
Can replace carcinogenic benzidine.

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