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L- Arginine 13C6 monohydrochloride, 100 mg, glass

≥98 % 13C
VE
Verp.
SILAC (Stable Isotope Labelling by amino acids in cell culture), 13C labelled Arg, (2S)-Amino-5-guanidino-n-valeric acid 13C6 hydrochloride, H-L-Arg-OH · HCl 13C6, 13C labelled arginine hydrochloride
Empirical formula 13C6H14N4O2 · HCl
Molar mass (M) 216,7 g/mol
WGK 1
CAS No. [201740-91-2]

For proteomics by mass spectrometry.

Type analysis

196,20 €/VE 

Excl. btw | 100 mg Per VE

Bestelnr. 2061.1

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Vanaf 6 VE 186,39 €/VE
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L- Arginine 13C6 monohydrochloride
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2061.1 100 mg glass 196,20 €
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Algemene informatie

Amino acids

Our amino acids are of the highest purity and suitable for a broad range of applications in biochemistry. Besides the natural occurring L‑amino acids, we offer a selection of unnatural D‑amino acids and DL‑amino acids.

Isotope Labelled Amino Acids (SILAC)

Stable Isotope Labelling with Amino Acids in Cell Culture (SILAC) is a metabolic labelling technique for mass spectrometric (MS)‑based quantitative proteomics. For this purpose originally identical cell cultures are cultivated with different nutrient media. One of the cell populations is fed with growth medium containing unlabelled amino acids. In contrast, the second cell culture is fed with a medium containing one or two amino acids labelled with stable (non‑radioactive) heavy isotopes. For example, the medium may contain arginine labelled with 13C carbon instead of the normal 12C carbon. The cells growing in this medium incorporate the heavy arginine into all their proteins. Then all peptides containing a single arginine are 6 Dalton heavier than their normal counterparts. The proteins from both cell populations are then mixed together in a ratio of 1:1 and analysed by mass spectroscopy. Pairs of chemically identical peptides of different isotopic compositions can be distinguished in a mass spectrometer on the basis of their mass difference. The ratio of the intensities in the mass spectrum for such peptide pairs reflects the frequency ratio for the two proteins.

References:
S.-E. Ong and M. Mann, A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC). Nature Protocols 2006, 1, 2650-2660.

Analysecertificaten

Hier kunt u uw analysecertificaat voor het geselecteerde product zoeken en downloaden. Geef een chargenummer op.
De volgende analysecertificaten werden gevonden:

Type analysis

Appearancewhite powder  
Assay (HPLC)≥95 %  
Isotope enrichment (13C)≥98 %  

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