Technical Data Sheet
ROTI®Fect, 5 ml, 5 x 1 ml
Ready-to-use products and reagents
For inquiries and further information, please contact our sales team
Storage temp. +4 °C
Transport temp. ambient temp.
WGK 1
Reagent for liposoma-mediated transfection of eukaryotic cells.
An efficient triple-cotransfection using ROTI®Fect is described in Ufartes, Schlüter, Kaulfuss and Seipp (2005) Biospektrum 4: 464-5.
€1,375.00/Pack Qty.
excl. VAT. | 5 ml per Pack Qty.
Art. No. P001.4
Product details
ROTI®Fect CELLPURE® ready-to-use, for transfection
ROTI®Fect is a liposome-based transfection reagent consisting of a mixture of polycationic and neutral lipids. Compared to monocationic lipid formulations, ROTI®Fect has a higher efficiency with lower levels being required. Its excellent reproducibility in combination with its high level of efficiency reduced optimisation work to a minimum. Because of its low toxicity, a wide range of cell lines can be transfected efficiently.
An efficient triple-cotransfection using ROTI®Fect is described in Ufartes, Schlüter, Kaulfuss and Seipp (2005) Biospektrum 4: 464-5.
- Highly efficient reproduction of DNA in mammal cells
- Excellent reproducibility
- High and wide operating plateau
- No inhibition through serum
- Low cell toxicity
- Successful with different cell lines
- Excellent transfection results
siRNA: CEM, COS-7, ES-D3,HeLa, HL60, LNCaP, OVCAR3, PC3, SW620
Primary cells: pAML, pHDF, pMEF, pRHC, pSMC
Cell lines: 143B, 293-T, A-293, A-431, AM-C6SC8, BHK, BV-2, C2C12, C6, CHO, CHO-DHFR, COS-1, COS-7, CRFK, Colo-205, DU-145, ECV-304, EMC, F98 (glioma), GD25-ß1, HBL 100, HCT-116, HCT-15, HEK293, HeLa, HeLa-S3, Hep-3B, Hep-G2, HOSE, HSG, HT-22, HT-29, HUVEC, JURKAT, LLC-PK1, LS174T, LoVo, MCF-7, MDCK, MeWo, MV3, NIH-3T3, NS20Y, OK, P815, PC3, PT-11, Rcho-1, SAOS, SH-SY5Y, SK-MEL-28, SKOV-3, SM10, SW-480, THP-1, tsA201, U87 (glioma), Vero 76, et al.
Approx. 400 assays per ml in 6 wells. Dependent on the cell line used.
Transfection of a variety of cell lines with ROTI®Fect and other commercially available transfectants.
Transfection was performed in 24wells using 0.25 µg DNA each, with 1.5 µl ROTI®Fect or acc. to instructions given by the manufacturers. Luciferase activity was measured 48 hours post transfection. Data of cells transfected with ROTI®Fect was set to 100 %.
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| Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
|---|---|---|---|---|---|---|
| P001.1 | 0.2 ml | plastic | 1 x 0,2 ml |
€92.90 |
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| P001.2 | 0.5 ml | plastic | 1 x 0,5 ml |
€199.00 |
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| P001.3 | 1 ml | plastic | 1 x 1 ml |
€309.00 |
|
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| P001.4 | 5 ml | plastic | 5 x 1 ml |
€1,375.00 |
|
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Available from stock Karlsruhe
Available (note delivery time)
In procurement
No longer available
Delivery date currently unknown
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