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ROTI®Garose-His/Ni Columns, 8 unit(s)

ROTI®Garose for biochemistry
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Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Danger
H225
i highly flammable liquid and vapour
P210 P280 P303+P361+P353
i keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking, wear protective gloves/protective clothing/eye protection/face protection, IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower
Related products
Pack Qty.
Pack.
IMAC columns, Nickel agarose beads, Nickel charged IMAC columns, Affinity chromatography colums
Flash point (flp) 17 °C
Storage temp. +4 °C
ADR 3 II
WGK 3
UN-Nr. 1170

For isolation of His-tagged proteins by affinity chromatography.
Prepacked colums for gravity flow chromatography.
Matrix volume 1 or 5 ml, whole volume 12 or 35 ml, respectively.
For approx. 100 and 500 mg His labelled protein.

Prepacked columns with nickel-loaded IDA agarose bead matrix for low-pressure affinity chromatography.

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort. ROTI®Garose His/Ni Columns offer the perfect solution for fast and simple isolation of His-tagged proteins from total lysates.
Product details
Type analysis

€265.00/Pack Qty. 

excl. VAT. | 8 unit(s) per Pack Qty.

Art. No. 1314.1

Available at short notice
from 6 Pack Qty. €251.75/Pack Qty.
from 24 Pack Qty. €238.50/Pack Qty.
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Product details



ROTI®Garose-His/Ni Columns ROTI®Garose for biochemistry

Prepacked columns with nickel-loaded IDA agarose bead matrix for low-pressure affinity chromatography.

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort. ROTI®Garose His/Ni Columns offer the perfect solution for fast and simple isolation of His-tagged proteins from total lysates.

  • Fast and simple purification of His-tagged proteins from total lysates
  • Superior recovery rate of pure His-tagged proteins
  • Binding capacity (purified 6xHis protein) of 117 mg per ml matrix
  • Easy elution and regeneration
  • Very low Nickel leaching
  • Compatible with denaturing reagents
  • For gravity flow chromatography

Suitable for

Suitable for all general applications, or if proteins of very small amount shall be isolated without loss.


Directions for use

ROTI®Garose His/Ni Columns are prepacked and may be used directly. The matrix consists of cross-linked and beaded 6 % agarose, IDA-conjugated and charged with divalent Nickel ions. Nickel chelates recognize two exposed histidines with good specificity and very high affinity, making the Ni2+ charged matrix the first choice for all standard applications. ROTI®Garose His/Ni Columns give very high yields of pure His-tagged protein with considerably low metal contamination. The tridentate IDA cross-linker provides easy elution with low amounts of imidazole. Additionally, ROTI®Garose Columns may repeatedly be regenerated, making them very cost-effective.

The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride, 5 mM DTT). Suspension in 20 % ethanol.
Column material made from polypropylene and polyethylene (frit).
May be autoclaved at 121 °C for 30 mins.



ROTI®Garose-His/Ni Columns
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1314.1 8 unit(s) cardboard €265.00
1314.2 5 unit(s) cardboard €685.00
Available from stock Karlsruhe
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Downloads

Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Particle size50-150 µm  
Beads (cross-linked agarose)6 %  
LigandIDA  
Metal ionnickel  
Binding capacity (DHAK-6xHis)117 mg/ml  
Activating agentEpichlorohydrin  
Stabilizer20 % ethanol  

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