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Transfer membrane ROTI®Nylon 0.2, Ø13.2 cm

From  ROTH SELECTION
Material: Nylon 6.6.
Dimensions


Ideal for DNA/RNA transfer, particularly for colony- and plaque-lifting, as well as for all multiple rehybridisations.
Especially recommended for Northern-blots.


ROTI®Nylon 0.2 is a neutral, enhanced Nylon membrane. Based on the material it provides a superior tensile strength and is extremely resistant against alcohol, acetone, and many other organic solvents.
Product details

€319.00/Pack Qty. 

excl. VAT. | 50 sheet per Pack Qty.

Art. No. HP39.1

Available from stock Karlsruhe
Delivery fast, simple and reliable!
from 6 Pack Qty. €303.05/Pack Qty.
from 24 Pack Qty. €287.10/Pack Qty.
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Product details

Material: Nylon 6.6.

Ideal for DNA/RNA transfer, particularly for colony- and plaque-lifting, as well as for all multiple rehybridisations.
Especially recommended for Northern-blots.


ROTI®Nylon 0.2 is a neutral, enhanced Nylon membrane. Based on the material it provides a superior tensile strength and is extremely resistant against alcohol, acetone, and many other organic solvents.


Directions for use

In a buffer pH of 6,5 ROTI®Nylon 0.2 is uncharged and by means of the surface-standing amino- and carboxy-groups has superior hydrophilic qualities. The charge of the membrane (ζ-potential) may be altered through the pH of the buffer - the membrane is positively charged at pH <6,5 and negatively charged at pH >6,5.
Membrane of particulary high DNA-binding capacity. Available as roll (pore size 0,2 µm) for high-flexible usage, and as disks (pore size 0,45 µm) for colony- and plaque-lifting. May be steam sterilised.
Covalent bondage of transferred nucleic acids is achieved by cross linking (baking for 15 min at 80 °C or UV-cross linking for 20 sec at 260 nm). Due to the excellent signal-to-noise ratio of the membrane, very sensitive detection (single copy-gene detection) and multiple rehybridisation is possible without loosing sensitivity.


Application examples

Southern- and Northern blot, Dot blot, DNA fingerprinting, ELISA, colony- and plaque lifting, macro- and microarrays.
Detection systems: radioactivity, chemoluminescence, fluorescence



Transfer membrane ROTI®Nylon 0.2 

Technical Information
Dimensions Ø13.2 cm 
Version disks 
Pore size 0,45 µm
Type 0,45 µm 
Load neutral/amphoteric 
Binding capacity 500 µg/cm²
Thickn. 150±20 µm
Immobilisation UV, baking 
Transfer membrane ROTI®Nylon 0.2
Selected quantity:   0
  1. Subtotal:  0.00
Order No. Dimensions Pack Qty. Price Quantity
AE50.1 300 × 30 cm 1 roll(s) €317.00
HP39.1 Ø13.2 cm 50 sheet €319.00
Available from stock Karlsruhe
Available at short notice
Not available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

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