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Methylene blue (C.I. 52015), 10 g

Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
i harmful if swallowed, harmful to aquatic life with long lasting effects
P270 P273
i do not eat, drink or smoke when using this product, avoid release to the environment
Pack Qty.
Basic blue 9
Empirical formula C16H18ClN3S
Molar mass (M) 319,9 g/mol
Melting point (mp) 190 °C
CAS No. [61-73-4]
EG-Nr. 200-515-2

For microscopy and staining of nucleic acids in polyacrylamide gels.
Dissolve 0.1-0.5 g in 100 ml of water

Watersoluble nuclear dye, does not intercalate between base pair stacks of DNA. Especially used for  ...
Product details
Type analysis

€19.50/Pack Qty. 

excl. VAT. | 10 g per Pack Qty.

Art. No. A514.1

Available from stock Karlsruhe
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Product details

Methylene blue (C.I. 52015) 

Watersoluble nuclear dye, does not intercalate between base pair stacks of DNA. Especially used for staining of blood cells.

Application note:

Staining of nucleic acids in PAA

Nucleic acids are visible as blue bands.

Detection limit: 20 ng/band.

  • Use a 0.2 % solution of methylene blue for staining
  • Stain for 20 min.
  • Wash for 15 min. in distilled water

Technical Information
Dyeing dead cells blue 
Ox. colour blue 
Red. colour colourless 
Redox potential E0[V] at pH=0 and 20 °C +0.53 (30 °C) 
Use vitality test 
Methylene blue (C.I. 52015)
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Order No. Pack Qty. Pack. Price Quantity
A514.1 10 g glass €19.50
A514.2 50 g glass €46.90
A514.3 100 g glass €75.90
Available from stock Karlsruhe
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General information

Determination of Surfactants

Benzethonium chloride solution is used to measure anionic detergents. In this method, the anionic detergent and the cationic detergent form a 1:1 salt in dichloromethane. The end point of the titration is indicated by methylene blue.

Indicators and Dyes

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Certificates of Analysis

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The following analysis certificates have been found:

Type analysis

Maximum absorption655-670 nm  
Loss on drying (110 °C)≤18,0 %  

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