dNTP-Set 1, 4 x 25 μmol
Storage temp. -20 °C
Transport temp. cooled
Concentration: 100 mM per dNTP
Typical end concentration in PCR: 200 µM each
€66.40/Pack Qty. Campaign price!
excl. VAT. | 1 set per Pack Qty.
Art. No. K039.1
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- Available as ready-to-use set or mix, for contamination-free applications
- Can be optimally combined with ROTI®Pol DNA polymerases from Carl ROTH
- Tested for “long range PCR“
- DNAse-, RNAse-, Protease- and Phosphatase-free
- Free of PCR inhibitors like modified bases and tetra-pyrophosphate
- Adjusted to pH 8,5 for superior stability even during larger numbers of freeze-and-thaw cycles
- Also available adjusted to pH 7,0 for special applications
- Highly efficient enzymatic fabrication of dATP, dGTP, dCTP and dUTP. dTTP ist synthesized chemically.
PCR, light-cycling, cDNA synthesis, labelling and primer-extension, mutagenesis-assays, sequencing reactions and in vitro transcription.
All Carl ROTH nucleotides are manufactured from highest-quality reagents and are most thoroughly tested for quality. This testing procedure not only includes standard-PCR but also ’long range PCR’, repeated quantitative light-cycling reactions, and tests for physical stability.
dNTP-Set 1 ≥99 %
Essai de sensibilité. Amplification d'un fragment de 260 bp sur un modèle de (de g. à d.) 250 ng (A), 25 ng (B) et 2,5 ng (C) d'ADN génomique humain (2 préparations chaque fois).
Sensitivity assay II: Quantitative light-cycling-PCR on recombinant DNA with (left to right) 10 ng, 1 ng, 100 pg, 10 pg, 1 pg DNA as template (six replicates).
Stability testing: HPLC analysis of all four dNTPs following incubation periodes of 1–14 days at different temperatures (4 °C – green; room temperature – red; 40 °C – blue). Stability of nucleotides is >99 % even after 14 days incubation at room temperature. Even after an incubation period of 9 days at 40 °C, 85 % of all nucleotide molecules are still intact.
- Subtotal: 0.00
|Order No.||Pack Qty.||Pack.||Packaging||Price||Quantity|
|K039.1||1 set||plastic||4 x 25 μmol||€66.40||
|K039.2||1 set||plastic||5 x 4 x 25 μmol||€274.75||
Available from stock Karlsruhe
Available at short notice
Delivery time on request