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Proteinase K - Solution, 5 ml

20 mg/ml, sterile, ready-to-use, for biochemistry and molecular biology
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Examples of effect: Have an allergenic effect, carcinogenic, mutagenic, toxic to reproduction and cause developmental toxicity (reprotoxic) or damage to organs Safety: You must be well-informed before starting work with these substances; wear protective clothing and gloves, eye protection and mask or breathing protection.
Danger
H317-H334
i may cause an allergic skin reaction, may cause allergy or asthma symptoms or breathing difficulties if inhaled
P280 P302+P352 P333+P313
i wear protective gloves/eye protection/face protection, IF ON SKIN: Wash with plenty of water, if skin irritation or rash occurs: Get medical advice/attention
Pack Qty.
Pack.
Density (D) 1 g/cm³
Boiling point (bp) 100 °C
Melting point (mp) 0 °C
Storage temp. -20 °C
Transport temp. cooled
WGK 1
CAS No. [39450-01-6]
EG-Nr. 254-457-8

Non-specific protease for degrading proteins in biological samples. Isolated from fungi (Tritirachium album).
Ready made solution

Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.
Product details
Guarantee analysis

€125.00/Pack Qty. 

excl. VAT. | 5 ml per Pack Qty.

Art. No. 3719.2

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Product details


Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.


Directions for use

Activity: (Haemoglobin, pH 7.5; 25 °C) 30 mAnson-U/mg
Foreign activity: RNase and DNase not detectable
Optimum temperature: +65 °C.
Activity at +65 °C is ca. 12 x higher than at +25 °C. Over +65 °C, inactivation due to denaturation.
Activators: Denaturating agents like SDS (0,5-1 %), urea.
Inhibitors: Inhibition with Hg2+-ions, DFP, PMSF and phenol. Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotryps ininhibitors.
Stability: pH 4.0-12.5. pH optimum: 8,0.
Also stable even when denaturing agents, e.g. SDS and urea are present.
Stabilisers: Ca2+-ions (1-5 mM) prevent autolysis.


Working concentration

Stock solution: 20 mg/ml in water
Storage temperature (stock solution): -20 °C
Reaction buffer: 50 mM Tris-HCl; pH 7.5; 5 mM CaCl2; 0.5 % SDS
Working solution: 50 µg/ml



Proteinase K - Solution 20 mg/ml, sterile, ready-to-use, for biochemistry and molecular biology

Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family. Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.

  • Sterile ready-to-use solution
  • Specific activity: ≥ 800 U/ml
  • Stable over a wide pH range: 4.0–12.5
  • Active at high temperatures and denaturing conditions


Proteinase K - Solution
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3719.1 1 ml glass €31.90
3719.2 5 ml glass €125.00
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Guarantee analysis

Appearanceclear, colorless solution  
Concentration20 mg/ml  
Specific activity≥800 U/mg  
Assay DNA≤0.25 pg/U  
Exonuclease activitynot detectable  
Endonucleasesnot detectable  

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