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Proteinase K, 100 mg

≥35 U/mg, BioScience Grade, lyophilised
Icon_DNAse-free
Icon_RNAse-free
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Examples of effect: Have an allergenic effect, carcinogenic, mutagenic, toxic to reproduction and cause developmental toxicity (reprotoxic) or damage to organs Safety: You must be well-informed before starting work with these substances; wear protective clothing and gloves, eye protection and mask or breathing protection.
Danger
H315-H317-H319-H334-H335
i causes skin irritation, may cause an allergic skin reaction, causes serious eye irritation, may cause allergy or asthma symptoms or breathing difficulties if inhaled, may cause respiratory irritation
P260 P280 P342+P311
i do not breathe dust, wear protective gloves/eye protection, if experiencing respiratory symptoms: Call a POISON CENTER/doctor
Pack Qty.
Pack.
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1
CAS No. [39450-01-6]
EG-Nr. 254-457-8

High-quality endopeptidase for molecular biology. Isolated from fungi (Tritirachium album).

Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.
Product details
Guarantee analysis
€165.00 /Pack Qty.

€128.70/Pack Qty.  Campaign price!

excl. VAT. | 100 mg per Pack Qty.

Art. No. 3726.1

Available from stock Karlsruhe
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Product details


Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.


Directions for use

Activity: (Haemoglobin, pH 7.5; 25 °C) 30 mAnson-U/mg
Foreign activity: RNase and DNase not detectable
Optimum temperature: +65 °C.
Activity at +65 °C is ca. 12 x higher than at +25 °C. Over +65 °C, inactivation due to denaturation.
Activators: Denaturating agents like SDS (0,5-1 %), urea.
Inhibitors: Inhibition with Hg2+-ions, DFP, PMSF and phenol. Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotryps ininhibitors.
Stability: pH 4.0-12.5. pH optimum: 8,0.
Also stable even when denaturing agents, e.g. SDS and urea are present.
Stabilisers: Ca2+-ions (1-5 mM) prevent autolysis.


Working concentration

Stock solution: 20 mg/ml in water
Storage temperature (stock solution): -20 °C
Reaction buffer: 50 mM Tris-HCl; pH 7.5; 5 mM CaCl2; 0.5 % SDS
Working solution: 50 µg/ml



Proteinase K ≥35 U/mg, BioScience Grade, lyophilised

Endopeptidase for the degradation of proteins with very high solubility, specific activity and DNA purity. Extra high purified.

Advantages:
  • High solubility
  • High specific activity
  • Extremely low DNA content

Activity: ≥ 35 U/mg lyophilizate
Specific activity: ≥ 45 U/mg protein
DNA content: ≤ 0.1 pg/mg



Proteinase K
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3726.1 100 mg glass €128.70
3726.2 1 g glass €584.20
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General information

Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)

There are six classes in which all enzymes are classified according to the particular reaction they catalyse:

Oxidoreductases (catalyse redox reactions)

Transferases (transfer functional groups among substrates)

Hydrolases (cleave bonds via addition of water)

Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)

Isomerases (transform chemical isomers)

Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Guarantee analysis

Appearancewhite powder  
Activity≥35 U/mg  
Specific activity≥45 U/mg  
Solubility in H2O≥50 mg/ml  
Assay DNA≤0.1 pg/mg  
Exonuclease activitynot detectable  
Endonuclease activitynot detectable  
Ribonucleases activitynot detectable  

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