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ROTI®Prep Gel Extraction, 50 preparations

for molecular biology
Examples of effect: Damage metals and burn body tissues; may cause serious eye damage. Safety: Avoid contact; wear safety spectacles and gloves. In the event of contact with eyes and skin, rinse with water.
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Warning
H290-H302+H312+H332-H315-H319-H412-EUH032
i may be corrosive to metals, harmful if swallowed, in contact with skin or if inhaled, causes skin irritation, causes serious eye irritation, harmful to aquatic life with long lasting effects
P280 P302+P352 P304+P340 P305+P351+P338 P312
i wear protective gloves/protective clothing/eye protection/face protection, IF ON SKIN: Wash with plenty of water, IF INHALED: Remove person to fresh air and keep comfortable for breathing, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, call a POISON CENTER/doctor if you feel unwell
Pack Qty.
Pack.
Packaging
Density (D) 1 g/cm³
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
ADR 9 III
UN-Nr. 3316

Kit for DNA extraction from agarose gels.
Yield up to 90 %, elution in minimum 10 μl.

DNA extraction from agarose gels through incubation and subsequent spin column process.
The gel slice to be extracted (up to 300 mg) is first solubilised in a lysis buffer through a simple 10 minute incubation. After that, DNA is elutied via convenient spin filter centrifugation in approx. 10 mins.
A specially developed Binding Enhancer is admixed in order to enhance binding efficiency of the DNA to the filter, and thus yield and purity of the isolated DNA.
Product details

€78.50/Pack Qty. 

excl. VAT. | 1 kit per Pack Qty.

Art. No. 8510.2

Available from stock Karlsruhe
Delivery fast, simple and reliable!
from 6 Pack Qty. €74.58/Pack Qty.
from 24 Pack Qty. €70.65/Pack Qty.
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Product details



ROTI®Prep Gel Extraction for molecular biology

DNA extraction from agarose gels through incubation and subsequent spin column process.
The gel slice to be extracted (up to 300 mg) is first solubilised in a lysis buffer through a simple 10 minute incubation. After that, DNA is elutied via convenient spin filter centrifugation in approx. 10 mins.
A specially developed Binding Enhancer is admixed in order to enhance binding efficiency of the DNA to the filter, and thus yield and purity of the isolated DNA.

  • Preparation by well-known mini spin-column system
  • Fast, easy and efficient
  • Extraction within approx. 20 minutes
  • For fragment length of 100 bp-30 kb
  • Recovery rate up to 90 %

Furthermore, the Roti®Prep Gel Extraction kit allows, if needed, to reduce elution volume down to 10 μl in order to obtain particularly highly concentrated DNA.
For gel runs both, TAE and TBE may be used. The kit is compatible with all standard gel loading buffers and gel staining reagents, even non-toxic ethidium bromide replacements like, for instance, Roti®GelStain.
Isolated DNA is free of residual agarose, PCR- and ligase inhibitors, and cytotoxic reagents, and can directly be applied to all standard downstream applications like cloning, PCR, transformation and transfection.

8510_RotiPrepGelExtraction_Ablaufschema

The kit contains

Binding Enhancer, Gel Solubizer, Washing Solution WSL, Elution Buffer EB, spin columns, collection tubes, elution tubes, detailed instructions-for-use.
Contents of this Kit may not be bought separately.

Please order ethanol 96-99,8 % (p.a., Art. No. 9065) separately.



ROTI®Prep Gel Extraction
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Order No. Pack Qty. Pack. Packaging Price Quantity
8510.1 1 kit cardboard 10 préparations €20.50
8510.2 1 kit cardboard 50 preparations €78.50
8510.3 1 kit cardboard 250 preparations €305.00
Available from stock Karlsruhe
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