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ROTI®Prep Plasmid MINI, 250 preparations

for molecular biology
Examples of effect: Damage metals and burn body tissues; may cause serious eye damage. Safety: Avoid contact; wear safety spectacles and gloves. In the event of contact with eyes and skin, rinse with water.
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Danger
H290-H302-H314
i may be corrosive to metals, harmful if swallowed, causes severe skin burns and eye damage
P280 P301+P312 P301+P330+P331 P303+P361+P353 P305+P351+P338
i wear protective gloves/eye protection, IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell, IF SWALLOWED: rinse mouth. Do NOT induce vomiting, IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water [or shower], IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Pack Qty.
Pack.
Packaging
DNA-Isolation kits
Density (D) 1 g/cm³
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
ADR 8 II
UN-Nr. 1760

Kit for fast and easy isolation of plasmid and cosmid DNA from recombinant bacteria.
Preparation of up to 20 μg plasmid DNA from up to 10 ml bacterial culture per column.

Coloured tracer for ideal DNA/membrane binding included.

Designed for the isolation of high-purity plasmid or cosmid DNA from bacteria, Roti®Prep Plasmid MINI Kit is based on the well-known "mini-prep" method, an alkaline lysis of bacteria followed by purification of the DNA from the cell debris, RNA and genomic DNA. The kit uses the easy-to-handle spin column technique, eliminating the need for time-consuming techniques like phenol-chloroform extraction and alcohol precipitation. The highly efficient and DNA-specific silica-gel membrane DNA absorption provides reliably high amounts of DNA, independently from vectors or bacterial strains used.
Product details

€269.00/Pack Qty. 

excl. VAT. | 1 kit per Pack Qty.

Art. No. HP29.2

Not available
from 6 Pack Qty. €255.55/Pack Qty.
from 24 Pack Qty. €242.10/Pack Qty.
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Product details



ROTI®Prep Plasmid MINI for molecular biology

Coloured tracer for ideal DNA/membrane binding included.

Designed for the isolation of high-purity plasmid or cosmid DNA from bacteria, Roti®Prep Plasmid MINI Kit is based on the well-known "mini-prep" method, an alkaline lysis of bacteria followed by purification of the DNA from the cell debris, RNA and genomic DNA. The kit uses the easy-to-handle spin column technique, eliminating the need for time-consuming techniques like phenol-chloroform extraction and alcohol precipitation. The highly efficient and DNA-specific silica-gel membrane DNA absorption provides reliably high amounts of DNA, independently from vectors or bacterial strains used.

  • Preparation by well-known mini spin-column system
  • Fast, easy and efficient
  • For all plasmids and cosmids of up to 10 kb length
  • Up to 20 μg highly pure DNA, directly applicable to restriction digest, sequencing etc.
  • 12 preparations in approx. 30 minutes
HP29_ComparisonOfYields
The figure depicts DNA of a 4 kb plasmid isolated by three equivalent kits
Lane 1, 2, 12, 13: Lambda Hind III Marker 500 and 750 ng, respectively
Lane 3, 4, 5: Kit competitor "I", 3 / 6 / 9 µl (1:10 dilution)
Lane 6, 7, 8: ROTI®Prep Plasmid MINI, 3 / 6 / 9 µl (1:10 dilution)
Lane 9, 10, 11: Kit competitor "II", 3 / 6 / 9 µl (1:10 dilution)

Up to 20 μg DNA can be isolated from up to 10 ml bacterial culture (5 preparations per column). DNA is free of RNA or protein contamination and can be directly applied to all standard down-stream applications like restriction digest, sequencing, in vitro transcription, transfection etc.


The kit contains

RNAse A (Art. No. 3953), Lysis buffer (Art. No. 3970), Neutralisation buffer (Art. No. 3971), Washing buffer (Art. No. 3975), Elution buffer (Art. No. 3978), Column activation buffer (Art. No. 3980), Mini spin columns (10/50/250), 2 ml Collection tubes (10/50/250), detailed instructions-for-use.
Excluding the columns, contents of this Kit may not be bought separately.

HP29_RotiPrepPlasmid_Ablaufschema


ROTI®Prep Plasmid MINI
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Order No. Pack Qty. Pack. Packaging Price Quantity
HP29.2 1 kit cardboard 250 preparations €269.00
HP29.4 1 kit cardboard 10 préparations €31.90
HP29.1 1 kit cardboard 50 preparations €69.50
Available from stock Karlsruhe
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