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ROTIPHORESE®10x TAE Buffer light

ROTIPHORESE® 10x conc., reduced EDTA concentration
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Pack Qty.
Pack.
TAE-buffer
Density (D) 1,018 g/cm³
Boiling point (bp) >100 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
WGK 1

Buffer concentrate for DNA agarose gel electrophoresis, ideal for DNA elution gels or in-gel-applications.
Concentrated TAE tends to precipitate salts during storage. Mix before use!

ROTIPHORESE®10x TAE-buffer light is particularly ideal for agarose gels in DNA ...
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Composition

€25.90/Pack Qty. 

excl. VAT. | 1 l per Pack Qty.

Art. No. 0122.1

Available from stock Karlsruhe
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ROTIPHORESE®10x TAE Buffer light ROTIPHORESE® 10x conc., reduced EDTA concentration

ROTIPHORESE®10x TAE-buffer light is particularly ideal for agarose gels in DNA-elution. Although the reduced EDTA-content lowers the contamination of the eluted DNA with EDTA and therefore prevents any interference of enzymatic activity, e.g. from ligases, isomerases or polymerases, the buffer properties still remain totally intact.
Particularly suitable for in-gel-applications when used with ROTI®Garose Low Melt (Art. No. 6351).

ROTIPHORESE®4 M Tris-acetate and 1 mM EDTA in distilled, deionised water; pH 8,3.


Directions for use

ROTIPHORESE®TAE bufferlight is usually used as 1x concentrated solution. TAE buffer is suitable for the separation of DNA fragments of all sizes in gels. Optimized for the run of gels for the gel extraction of DNA.


Mechanism

TAE buffer has a lower buffer capacity than TBE and can be used with higher voltages, longer gels and larger DNA fragments. The gels heat up during running to a lesser extent than when using the running buffer TBE. DNA migrates in TAE gel about twice as fast as in TBE buffered gels.



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Composition

0.4 M TRIS acetate., 1 mM EDTA., distilled, deionized water., Remark: The product may crystallise. Can be redissolved by heating (max. 40 °C).

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