Jump to content Jump to navigation menu

HEPES, 1 kg

≥99,5 %, BioScience Grade
Icon_DNAse-free
Icon_Protease-free
Icon_RNAse-free
Related products
Pack Qty.
Pack.
N-2-Hydroxyethylpiperazine-N'-2-ethane sulphonic acid, Good's buffer
Empirical formula C8H18N2O4S
Molar mass (M) 238,31 g/mol
Flash point (flp) 124 °C
Melting point (mp) 212,6 °C
WGK 1
CAS No. [7365-45-9]
EG-Nr. 230-907-9

For molecular biology and biotechnology.

HEPES is a versatile buffer, which is zwitterionic over a broad pH-range, interesting for biological assays, and, therefore, doesn't pass through biological membranes. In contrast to NaHCO3 buffers, it also doesn't release any CO2 which affects the surroundings and has to be compensated. Hence, HEPES is particularly adapted for cell- and tissue cultures.

HEPES cannot be autoclaved and reacts with DEPC. Thus it's recommended to use DNase/RNase/Protease free HEPES BioScience-Grade as soon as molecular biological applications like PCR, extraction, isolation, hybridization etc. are to be performed.
Product details
Guarantee analysis
€232.35 /Pack Qty.

€181.20/Pack Qty.  Campaign price!

excl. VAT. | 1 kg per Pack Qty.

Art. No. 6763.3

Available from stock Karlsruhe
Delivery fast, simple and reliable!
Carl Roth subscription service

Now recurring orders conveniently delivered as a subscription!

With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!

How it works:
1

Put all products for your subscription in the desired quantity in the basket.

2

In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.

3

Set Starty point and interval for your subscription and submit order!

By the way: Through your account you can customize or delete your subscriptions anytime.

Product details



HEPES ≥99,5 %, BioScience Grade

HEPES is a versatile buffer, which is zwitterionic over a broad pH-range, interesting for biological assays, and, therefore, doesn't pass through biological membranes. In contrast to NaHCO3 buffers, it also doesn't release any CO2 which affects the surroundings and has to be compensated. Hence, HEPES is particularly adapted for cell- and tissue cultures.

HEPES cannot be autoclaved and reacts with DEPC. Thus it's recommended to use DNase/RNase/Protease free HEPES BioScience-Grade as soon as molecular biological applications like PCR, extraction, isolation, hybridization etc. are to be performed.


Buffer reagent according to Good. High buffer capacity at physiological pH-values.



Technical Information
pH range 6.8 to 8.2 
Buffer reagent N-2-Hydroxyethyl piperazine-N'-2-ethane sulphonic acid, Good's buffer 
HEPES
Selected quantity:   0
  1. Subtotal:  0.00
Order No. Pack Qty. Pack. Price Quantity
6763.1 100 g plastic €28.45
6763.2 500 g plastic €109.55
6763.3 1 kg plastic €181.20
Available from stock Karlsruhe
Available at short notice
Not available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Guarantee analysis

Assay≥99,5 %  
Solubility (0,1 M in H2O)clear, complete  
pKa (at 25 °C)7,45-7,65  
Absorbance
260 nm≤0,02  
280 nm≤0,02  
405 nm≤0,01  
Chloride (Cl)≤0,005 %  
Sulphate (SO4)≤0,01 %  
Phosphate (PO4)≤0,001 %  
Calcium (Ca)≤0,003 %  
Sodium (Na)≤0,005 %  
Heavy metals (as Pb)≤0,0005 %  
DNases, RNases, Proteasenone detected  

0 Reviews

0.0 out of five stars