• Highly reliable
• Easy to perform
• Fast detection procedure (only 30 min)
• Mild conditions (no DNA denaturation required)
• Modular system due to click chemistry
• Compatible with various dyes and readouts
• Compatible with multiplexing
• No cytotoxicity up to 1 mM EdU

Our ROTI^®Kits for EdU mediated detection of cell proliferation provide a superior alternative to time-consuming and less sensitive bromodeoxyuridine (BrdU) assays. Similar to BrdU assays, our assays utilise a uracil derivative (in this case ethynyldeoxyuridine, EdU) which is incorporated into the replicating DNA. EdU is not detected via antibodies; instead fluorescent dyes are coupled directly via click reaction. This fast and elegant approach allows for direct labelling of replicating DNA with the desired fluorescent dye. Thus, the assay is perfectly adaptable to the present facilities and to the assay situation (e.g. for double or triple detection). Furthermore, the click reaction is highly selective and therefore prevents any unspecific labelling. The protocol includes only few steps and decreases the total amount of time.

Figure: Proliferating tissue of barley meristem labelled via EdU Click-555 kit (Art. No. 7775.1). Dividing cells incorporated EdU and were labelled via click reaction with TAMRA (pink stained cells). Nuclei were counterstained with DAPI (blue). Magnification: 100x; Filterset: Sp. Aqua HC mFISH; Sp. Green HC mFISH.

The number at the end of the product name specifies the wavelength at which the included fluorescent marker is optimally excited.