• Alternative for ethidium bromide, non-toxic, non-mutagen
• Nearly as sensitive as ethidium bromide, up to 1,5 ng/mm² nucleic acid
• For use with common broad-band ethidium bromide photo filters
• Excitation possible via UV light (320 nm) and blue light (~470 nm)
• Compatible with all usual down-stream applications

New, versatile reagent for gel loading and simultaneous staining of double stranded nucleic acid in agarose- and polyacrylamide gels.

Roti^®-Load DNAstain detects up to 0,2 ng per band, and is, therefore, as sensitive as ethidium bromide. Due to its most simple application as gel loading buffer it reduces the time needed for gel analysis significantly. This innovative reagent contains the dye EvaGreen^®, which has been developed specifically for DNA analysis. DNA integrity is not influenced by EvaGreen^®; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligation, PCR, transformation, transfection etc. without interfering effects. Due to the low membrane permeability of the dye, Roti^®-Load DNAstain is non mutagenic, non toxic, and environment friendly.
Roti^®-Load DNAstain can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also Rotiphorese^® PROfessional runVIEW, Art. No. 4849.1). When excited, nucleic acid-bound Roti^®-Load DNAstain emits a brightly coloured green fluorescence (525 nm) that may be documented by all usual broadband ethidium bromide- or SYBR^® Green foto filters.

The figure depicts 50 ng DNA per band, or 75 ng (1000 bp and 2,5 kb), respectively. Each lane has been loaded and concurrently stained using 1 µl Roti^®-Load DNAstain.

Roti^®-Load DNAstain 3 contains: Tris, EDTA, orange G, EvaGreen^®.

Excitation maximum (bound to DNA): approx. 320 nm and 470-500 nm

Emission maximum (bound to DNA): 525 nm

Recommended for staining of dsDNA- and dsRNA-fragments of under 500 bp.