Przejdź do treści Przejdź do menu nawigacyjnego

ROTI®Lyte pH 5-6, 10 ml

40 % in water, sterile filtered (0,2 μm)
Icon_Steril Sterile products
Produkty pokrewne
Pack Qty.
Pack.
Ampholyte, Carrier Ampholyte
Density (D) 1,12 g/cm³
Storage temp. +4 °C
WGK 1

Carrier ampholytes for isoelectric focussing (IEF).
Added in 3-5 % working concentration to acrylamide- or agarose gel solution.

Type analysis

204,50 €/op. 

plus VAT | 10 ml za op.

Nr kat. 5851.1

Dostępny z magazynu w Karlsruhe
Szybka, prosta i niezawodna dostawa.
od 6 op. 194,28 €/op.
od 24 op. 184,05 €/op.
Usługa abonamentowa firmy Carl Roth

Now recurring orders conveniently delivered as a subscription!

With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!

How it works:
1

Put all products for your subscription in the desired quantity in the basket.

2

In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.

3

Set Starty point and interval for your subscription and submit order!

By the way: Through your account you can customize or delete your subscriptions anytime.

Czy masz pytania do tego produktu?

Do you have a question about this product?

In the general information or Product details, we have compiled extensive descriptions and downloads for each product.
If you can not find an answer to your question in the Certificates of Analysis and Application Examples , feel free to contact us!
Contact Service

Szczegóły dotyczące produktu


  • Reliable formation of a finely graded linear pH gradient
  • Sharp band resolution due to high buffer capacity at pI
  • High conductivity, no overheating of the gel
  • Short staining- and destaining periods
  • Compatible with all standard staining methods

ROTI®Lyte carrier ampholytes consist of a diverse mixture of sterile filtered hydrous polyamino-polysulfone-polycarboxylic acids. Due to the multitude of isoelectric points (pIs) present, those amphoteric molecules form a very finely graded, linear pH gradient in the electric field, hence making them particularly suitable for isoelectric focusing (IEF).

ROTI®Lyte carrier ampholytes excel by their high buffer capacity and conductivity, leading to excellent band definition. Even proteins differing by just 0.01 pH units may be discriminated efficiently. Due to their low molecular weight of 1000 Da in maximum, the ampholytes disperse quickly in the gel, therefore guaranteeing an even distribution of sample proteins during IEF. Since there is virtually no unspecific binding of dyes, no background staining happens, and ROTI®Lyte ampholytes are perfectly compatible with all standard staining methods. They are well soluble in trichloroacetic acid, and may easily be removed from the gel during subsequent fixation.


Directions for use

Store unopened bottles at +4 °C. Opened bottles should be aliquoted and stored at -20 °C. Avoid frequent freeze and thaw cycles.



ROTI®Lyte pH 5-6 40 % in water, sterile filtered (0,2 μm)

5841_Bande_ai
Figure (example 5841.1):
pH range 3-10, gel thickness 350 µm
Lanes 1, 2, 5, 6: Potato extracts (10 µl)
Lanes 3, 4: Protein standard (10 µl)


Informacje techniczne
Conductivity 0,05-0,5  mS/cm
pH upper limit 6,2 ±0,5  
pH lower limit 5,3 ±0,3  
ROTI®Lyte pH 5-6
Wybrana ilość:   0
  1. Suma pośrednia:  0.00
Nr kat. Pack Qty. Pack. Cena Ilość
5851.1 10 ml glass 204,50 €
5851.2 25 ml glass 449,00 €
Dostępny z magazynu w Karlsruhe
Dostępny krótkoterminowo
Niedostępny
Data dostawy obecnie nieznana
Wybrana ilość:   0
  1. Suma pośrednia:  0.00

Pobranie

Świadectwa jakości

Tu możesz wyszukać i pobrać świadectwo jakości wybranego produktu. Wpisz numer serii.
Znaleziono następujące świadectwa jakości:

Type analysis

Appearanceclear solution  
Content (w/v)40 %  
pH-value (2.0 % in H2O, 20 °C)5,3-5,7  
Conductivity (2,0 % in H2O, 20 °C)0,05-0,50 mS/cm  
pH Range5,3 ±0,3 to 6,2 ±0,5  
pH gradient performedmin. 70 %