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ROTI®Pol Hot-TaqUltra, 200 µl, 5 x 200 U

5 U/μl, DNA-free
Icon_Eisstern All products identified as being particularly temperaturesensitive are shipped in special ice boxes with freezer packs or in dry ice.
Pack Qty.
Pack.
Packaging
DNA polymerase (thermostable), Hot start DNA polymerase, Taq DNA polymerase, recombinant, Polymerase (thermostable)
Storage temp. -20 °C
Transport temp. cooled
WGK 1

For hot start PCR amplifications, particularly of bacterial DNA.
Ultra pure HotTaq polymerase in storage buffer

For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.
Szczegóły dotyczące produktu
Type analysis

735,00 €/op. 

plus VAT | 200 µl za op.

Nr kat. 9350.2

Dostępny z magazynu w Karlsruhe
Szybka, prosta i niezawodna dostawa.
od 6 op. 698,25 €/op.
od 24 op. 661,50 €/op.
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Szczegóły dotyczące produktu


For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.


ROTI®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.



ROTI®Pol Hot-TaqUltra 5 U/μl, DNA-free

Hot start version of the recombinant heat stable Taq DNA polymerase from the thermophilic bacterium Thermus aquaticus in storage buffer and tested on the absence of bacterial DNA. ROTI®Pol Hot-TaqUltra is recommended for use in highly specific PCR applications in general, or if bacterial DNA, or in RT-PCR, bacterial 16S rRNA shall be detected.
ROTI®Pol Hot-TaqUltra is purified using a multiple-step process that minimizes contaminating bacterial DNA to a none detectable level. Each lot of the polymerase undergoes strict quality control testing in order to ensure the absence of detectable amounts of contaminating bacterial DNA. ROTI®Pol Hot-TaqUltra is provided in a DNA-free storage buffer containing 50 % glycerol.
ROTI®Pol Hot-TaqUltra is recommended for use when particularly specific amplification of bacterial DNA is the main focus. The antibody-mediated blocking of the DNA polymerase is released only at the initial denaturation step, hence resulting in highly specific amplification of the target sequence without production of unwanted side products caused by unspecific primer annealing.
ROTI®Pol Hot-TaqUltra is able to amplify PCR products up to 5 kb and is appropriate for use in the amplification of DNA from eukaryotic as well as prokaryotic templates. The Hot-TaqUltra DNA polymerase possesses a 5’ → 3’ polymerase- as well as a 5’-3' exonuclease activity, and generates a 3'dA (adenine)-overhang which may well be used for TA-cloning purposes.


For research use only. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro diagnostics.



For research use only. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro diagnostics.
Informacje techniczne
Application Particularly sequence specific PCR of bacterial gDNA 
Amplicon ends 3'dA 
Polymerase DNA free, ultra pure hot start Taq polymerase 
Use Particularly sequence specific PCR of bacterial gDNA 
ROTI®Pol Hot-TaqUltra
Wybrana ilość:   0
  1. Suma pośrednia:  0.00
Nr kat. Pack Qty. Pack. Packaging Cena Ilość
9350.1 40 µl plastic 1 x 200 U 154,50 €
9350.2 200 µl plastic 5 x 200 U 735,00 €
Dostępny z magazynu w Karlsruhe
Dostępny krótkoterminowo
Niedostępny
Data dostawy obecnie nieznana
Wybrana ilość:   0
  1. Suma pośrednia:  0.00

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Type analysis

Purity (enzyme)≥98%  
Enzyme activity5 U/µl  
Endonuclease activitynone detected  
Exonuclease activitynone detected  
Suitability for PCR (400 bp)complies  
Suitability for PCR (3 kb)complies  
Bacterial DNA (enzyme)none detected  
Selective specificity in PCRcomplies  
Hot start functionalitycomplies  
Stability (in storage buffer)complies  
For research use only. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro diagnostics.